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Anti-alpha-Fetoprotein Purified

Antibodies Primary

Make a customized order

Article No

AFC-IF767E-0.1

Species Reactivity

human

Size

0.1 mg

Clone

AFP-01

Source / Host

mouse

Application

IP, ELISA, WB, ICC

Article No

AFC-IF767E-0.1

Species Reactivity

human

Size

0.1 mg

Clone

AFP-01

Source / Host

mouse

Application

IP, ELISA, WB, ICC

Specifications
Suggested protocols
References

Specifications

Application	IP, ELISA, WB, ICC
Article No	AFC-IF767E-0.1
Biosite Brand	Biosite Flow
Country Availability	all
Clone	AFP-01
Clone Type	monoclonal
Concentration	1 mg/ml
Conjugation	Unconjugated
Description	Anti-alpha-Fetoprotein Purified
Supplier	Nordic Biosite
Entrez Gene ID	174
Format	Purified
Immunogen	purified alpha-Fetoprotein
Isotype	IgG1
Keywords	Serum & Plasma Proteins (Human)
Notes	Alpha-fetoprotein (AFP) is present in fetal plasma, and it binds e.g. copper, nickel, and bilirubin. Measuring of alpha-fetoprotein level in amniotic fluid can reveal severe fetal defects. In adults, elevated AFP concentrations in the plasma can indicate hepatocellular carcinoma or teratoblastoma. In some individuals, hereditary persistance of alpha-fetoprotein can be observed without any obvious pathology.
Alias Names	AFP, FETA, HPAFP
Previous Article No	AFC-4205-2, AFC-IF767E-0.1
Product Type	Antibodies Primary
Protocol	ELISA: The antibody AFP-01 has been tested as the detection antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-11 (cat. no. 11-384-C100). Western blotting: Positive material: Hep G2 human hepatocellular carcinoma cell line; both non-reducing and reducing conditions. Immunoprecipitation: Interaction of the antibody AFP-01 with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions.
Purification	Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Purity	> 95% (by SDS-PAGE)
Size	0.1 mg
Source / Host	mouse
Species Reactivity	human
Storage	Store at 2-8°C. Do not freeze.
Substrate / Buffer	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Technical Specifications	The mouse monoclonal antibody AFP-01 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
UniProt Number	P02771
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

ELISA

Immunoprecipitation

Western Blot

References

*Stefanova I, Horejsi V, Kristofova H, Angelisova P, Zizkovsky V, Hilgert I: Monoclonal antibodies against human alpha-fetoprotein. Exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes. J Immunol Methods. 1988 Jun 28;111(1):67-73., URL: https://www.ncbi.nlm.nih.gov/pubmed/2455753?dopt=A,*Cavin LG, Venkatraman M, Factor VM, Kaur S, Schroeder I, Mercurio F, Beg AA, Thorgeirsson SS, Arsura M: Regulation of alpha-fetoprotein by nuclear factor-kappaB protects hepatocytes from tumor necrosis factor-alpha cytotoxicity during fetal liver development and hepatic oncogenesis. Cancer Res. 2004 Oct 1;64(19):7030-8. , URL: https://www.ncbi.nlm.nih.gov/pubmed/15466196?dopt=,*Cermák L, Símová S, Pintzas A, Horejsí V, Andera L: Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8;277(10):7955-61., URL: http://www.ncbi.nlm.nih.gov/pubmed?term=%C4%8Derm%C3%A1k%20l%20%C5%A1%C3%ADmov%C3%A1%20%C5%A1%20pintzas%20a ,*Green CJ, Charlton CA, Wang LM, Silva M, Morten KJ, Hodson L: The isolation of primary hepatocytes from human tissue: optimising the use of small non-encapsulated liver resection surplus. Cell Tissue Bank 2017 Dec;18(4):597-604., URL: https://pubmed.ncbi.nlm.nih.gov/28717878/,*Colquhoun DR, Goldman LR, Cole RN, Gucek M, Mansharamani M, Witter FR, Apelberg BJ, Halden RU: Global screening of human cord blood proteomes for biomarkers of toxic exposure and effect. Environ Health Perspect. 2009 May;117(5):832-8., URL: https://pubmed.ncbi.nlm.nih.gov/19478969/,*Wang Z, Bi B, Song H, Liu L, Zheng H, Wang S, Shen Z: Proliferation of human hepatocellular carcinoma cells from surgically resected specimens under conditionally reprogrammed culture. Mol Med Rep. 2019 Jun;19(6):4623-4630., URL: https://pubmed.ncbi.nlm.nih.gov/31059040/,,,

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