Science Hub

Life Science Blog

Welcome to Nordic BioSite's Life Science Blog. Here, we will provide commentary on current events and trends in biological sciences, interesting stories about important scientists, technical tips and suggestions, and much more. Read on and learn something new.

Product Highlights: Immune Checkpoints

Immune checkpoints regulate when and how the immune system is activated. Their correct regulation and function are critical for maintaining a self-tolerant immune system that knows when to act (i.e. during infections, cancer) and when not to (i.e. to prevent autoimmunity). Checkpoints are maintained by a large group of cell-surface receptors, including stimulatory and inhibitory checkpoint molecules that together fine-tune immune signalling.

Immunohistochemistry Image Analysis – an Image Paints a Thousand Words

In our last article we looked at the workflow for a typical immunohistochemistry (IHC) experiment. Regardless of the tissue type under investigation, the target biomolecules, and the detection method chosen, it is necessary to apply a method to extract and analyse the information held on the stained slides. The detection method used will influence image analysis to some extent i.e. light microscopy or fluorescence microscopy is used to visualise slides after chromogenic or fluorescence detection, respectively. With the right equipment, it is possible to scan entire slides for easier viewing of entire tissue sections, further analysis and to get high-quality images in digital format for use in publications and presentations.

RNA-Seq 4: Planning Your Experiment

Welcome back to the RNA-seq series! In this article, we will go through some of the main parameters you will need to consider when setting up a new RNA-seq experiment!

The Typical Immunohistochemistry Workflow

Immunohistochemistry (IHC) ties together immunological, biochemical and microscopic techniques to yield visual information about proteins and other macromolecules in tissues. Targets are detected through the use of labelled antibodies that penetrate tissues and specifically bind proteins and other cellular components of interest, e.g., organelles.

RNA-Seq 3: Technology Overview

Welcome back to our RNA-seq article series! Now that we’ve gone through the general workflow and the ins and outs of RNA quality, we will look at some of the popular methods and platforms that you are likely to encounter if you embark on RNA-seq experiments.

Parkinson’s Disease & The Microbiome

Parkinson’s disease (PD) is a debilitating and incurable age-related neurodegenerative disease that affects bodily movements and worsens over time. It is one of the most common neurological diseases and its incidence is rising rapidly, with 14 million cases expected globally just 20 years from now.