Science Hub

Welcome to Nordic BioSite's Life Science Blog. Here, we will provide commentary on current events and trends in biological sciences, interesting stories about important scientists, technical tips and suggestions, and much more. Read on and learn something new.
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DNA Sample Collection and Isolation

One of the ever-intriguing question most of us ask is ‘who we are’ or ‘where do we come from’? To answer this simple yet challenging question one should focus on to our genome. Dr. Svante Pääbo from his groundbreaking research have contributed immensely to our understanding of the human evolution for which he was awarded Nobel prize in Physiology or Medicine 2022. He and his team from years of research was able to shed light on human evolution which have created an entirely new field of genomics termed Paleogenomics.

World Heart Day

Today is the annual World Heart Day, which is held every year on the 29th of September, and which is the world’s biggest awareness-raising platform for cardiovascular disease (CVD), a class of diseases that affect the heart or blood vessels, including heart disease and stroke.

7 things to consider before using the Immunofluorescence technique

Immunofluorescence (IF) technique as the name suggest is the use of antibodies to target and visualize protein in microbiological samples, cells and tissues. Knowledge of protein localization is important to understand their cellular function that together with other immunological and molecular biological assays provides information in terms of health and disease.

Multiplex Real-Time PCR for Beginners

This post was originally posted on June 3rd 2020.

In case the name doesn’t give it away, multiplex real-time PCR involves amplifying multiple DNA or RNA targets simultaneously in a single PCR reaction. This requires the presence of a specific pair of primers and a complementary DNA-binding probe for each target under study.

Restriction Enzymes 101

This post was originally posted on October 28th 2020.

Restriction enzymes or restriction endonucleases recognise short, specific DNA sequences known as restriction recognition sites, and make double-stranded cuts at or adjacent to these sites. These enzymes are naturally occurring in bacteria and archaea where they function as an ancient sequence-directed immune system, chopping up phage DNA at restriction sites in the genome of the invading phage.

Next Generation Sequencing 101 – Part 2

Welcome back to our next generation sequencing (NGS) 101 mini-series! In Part 1, we introduced the basics of NGS and provided an overview of some of its major applications. In this follow-up, we take a look at the typical NGS workflow including tips for good sample collection and NGS library preparation.

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