Science Hub

Life Science Blog

Welcome to Nordic BioSite's Life Science Blog. Here, we will provide commentary on current events and trends in biological sciences, interesting stories about important scientists, technical tips and suggestions, and much more. Read on and learn something new.

The Lowdown on Real-Time PCR – Part 2

Welcome to part 2 of our real-time PCR series. In part 1, we went through the basics of real-time PCR, its advantages over end-point PCR, a typical workflow, data output, and the choice of fluorescent labeling systems available.

In part 2, we take a look at the different quantification methods available, setup tips, primer design and quality control.

The Lowdown on Real-Time PCR – Part 1

In case the name doesn’t give it away, real-time PCR is a PCR application that monitors DNA amplification in real time. This means that amplification is monitored during the PCR reaction, and not at the end of the reaction as with end-point PCR, where PCR products are typically analyzed post-run on agarose gels.

Nordic BioSite’s attending the 45th SSI meeting in Geilo

Nordic BioSite will be at the 45th Scandinavian Society for Immunology (SSI) meeting, taking place in beautiful Geilo, Norway 2nd-6tf of April. You can come and talk to us during 3 days filled with exciting scientific speakers and information about the newest products for immunology research

VeriCells™ – Control Cells for Flow Cytometry

Everyone loves good flow cytometry data, but having the right controls to interpret and troubleshoot your experiment is just as critical. Whether you need to accurately gate your populations or figure out whether your result is unique or an artifact, appropriate controls provide context and clarity. Flow cytometry controls are of particular importance in reference laboratories, clinical research organizations, and multi-center clinical trials to monitor assay performance and variability for longitudinal studies.

ELISA Principles 101

ELISA (Enzyme Linked Immuno-Sorbent Assay) is a highly sensitive immunoassay for in vitroquantification of soluble analytes, including: cytokines, CD antigens, apoptosis markers, hormones, metabolites, and growth factors.

Simplify Your Multiomics Workflow with Zymo’s Co-Purification Kits

Genomes, epigenomes, transcriptomes, proteomes, and metabolomes. The list goes on…

In this rapidly evolving ‘omics’ era, researchers are generally becoming less enthusiastic about analyzing a genome, a transcriptome, or a proteome, etc., in isolation. More and more, researchers want to study multiple ‘omes’ in parallel, and one of the most popular combinations nowadays is genome and transcriptome analysis from the same sample.

Planning In Vivo Functional Assays? Look No Further for In Vivo Antibodies!

Besides target staining/detection and quantification, for example, via Western blotting, immunohistochemistry, and flow cytometry, antibodies can be used for a variety of functional assays such as:

  • Blocking – where an antibody binds its target, thus directly interfering with its function, for example, by blocking its ability to bind a ligand.
  • Neutralization – here, an antibody binds its target and abolishes its downstream effects, for example, its ability to influence cell proliferation.
  • Activation or agonism – this may occur when an antibody binds a receptor in a manner that mimics the binding of the receptor’s natural ligand, resulting in antibody-mediated downstream signaling.
  • Immunodepletion – removal of a target protein by sequestering it with an antibody.

Finally! Link RNA and Protein Expression Levels with TotalSeq™ from BioLegend

In recent years, the field of multiomics has undergone an increasing expansion of interest from the scientific community. Several techniques have been developed to gain an increase in resolution when studying cellular characteristics. Using multiomics technology, scientists can now rebuild cell lineage trajectories to further understand animal development, to unravel how cancer cells develop during carcinogenesis by linking mutations with cellular behavior, and to identify cell subtypes within a population of cells (Reviewed in 1).

Beginner’s Guide to Immunohistochemistry 1: Choosing a Primary Antibody

As with any other experimental setup, there are many factors to consider when embarking on a new immunohistochemistry (IHC) project, for example, the kind of tissue type you will stain and whether it will be fixed or frozen, the labelling/detection system, suitable staining controls, and the way in which you will analyse your data, to name a few.

DNA/RNA Shield™ for Next-Generation DNA & RNA Sample Preservation

Nowadays, the sky is the limit when it comes to the number of ways we can investigate our favorite organism or cell type. ‘Omics’ approaches such as genomics, transcriptomics, epigenomics, metagenomics, and metatranscriptomics have the power to generate massive amounts of valuable information that can help us to better understand biological processes, develop new diagnostic tools, and find new ways to treat diseases.

Sample Collection and Preservation – Critical Starting Points in Your Research!

In the current ‘omics’ era, there seems to be no limit to the number of ways we can investigate our favorite organism or cell type. We can study genomes, transcriptomes, epigenomes, metabolomes, proteomes, kinomes, and more. Parallel advances in flow cytometry and other immunological techniques make it possible for us to simultaneously detect and profile new and characterized cell surface markers in robust multiplex setups.