Science Hub

Life Science Blog

Welcome to Nordic BioSite's Life Science Blog. Here, we will provide commentary on current events and trends in biological sciences, interesting stories about important scientists, technical tips and suggestions, and much more. Read on and learn something new.

RNA-Seq 1: Introduction

RNA sequencing or RNA-seq uses next-generation sequencing (NGS) technology to provide a snapshot of the numbers and identities of RNA molecules in any sample at any time under a condition(s) of interest. Since its inception in the early 00’s until now, data gleaned from RNA-seq experiments has revolutionised our understanding of RNA, its roles in animal and plant development, the importance of differential gene expression during health and disease, and how individuals respond to drug treatments. It has also revealed that eukaryotic transcriptomes are much more complex than previously thought!

Genotyping for Beginners

Genotyping encompasses the detection or mapping of small genetic differences between members of the same species and across different species. It is often used to gain insight into how genetic variation leads to phenotypic changes, including those that underpin the physical differences between individuals (e.g., human appearance) as well as those that manifest as disease. Other uses include epidemiology, forensics, plant breeding and ancient DNA analysis.

Beginner’s Guide to Immunohistochemistry 2: Choosing a Secondary Antibody

Welcome back to our beginner’s guide to immunohistochemistry (IHC). In this post, we’ll follow on from our last article on choosing a primary antibody, bringing you the most important considerations for choosing a secondary antibody.

Sample Collection and Preservation – Critical Starting Points in Your Research!

In the current ‘omics’ era, there seems to be no limit to the number of ways we can investigate our favorite organism or cell type. We can study genomes, transcriptomes, epigenomes, metabolomes, proteomes, kinomes, and more. Parallel advances in flow cytometry and other immunological techniques make it possible for us to simultaneously detect and profile new and characterized cell surface markers in robust multiplex setups.

Zombie Fixable Viability Dyes – When It’s a Matter of Life and Death

In any experiment, the quality of your data will be influenced to a large extent by the quality of the sample(s) used. Flow cytometry is no exception to that rule.

The Lowdown on Real-Time PCR – Part 2

Welcome to part 2 of our real-time PCR series. In part 1, we went through the basics of real-time PCR, its advantages over end-point PCR, a typical workflow, data output, and the choice of fluorescent labeling systems available.

In part 2, we take a look at the different quantification methods available, setup tips, primer design and quality control.

The Lowdown on Real-Time PCR – Part 1

In case the name doesn’t give it away, real-time PCR is a PCR application that monitors DNA amplification in real time. This means that amplification is monitored during the PCR reaction, and not at the end of the reaction as with end-point PCR, where PCR products are typically analyzed post-run on agarose gels.

Nordic BioSite’s attending the 45th SSI meeting in Geilo

Nordic BioSite will be at the 45th Scandinavian Society for Immunology (SSI) meeting, taking place in beautiful Geilo, Norway 2nd-6tf of April. You can come and talk to us during 3 days filled with exciting scientific speakers and information about the newest products for immunology research

VeriCells™ – Control Cells for Flow Cytometry

Everyone loves good flow cytometry data, but having the right controls to interpret and troubleshoot your experiment is just as critical. Whether you need to accurately gate your populations or figure out whether your result is unique or an artifact, appropriate controls provide context and clarity. Flow cytometry controls are of particular importance in reference laboratories, clinical research organizations, and multi-center clinical trials to monitor assay performance and variability for longitudinal studies.

ELISA Principles 101

ELISA (Enzyme Linked Immuno-Sorbent Assay) is a highly sensitive immunoassay for in vitroquantification of soluble analytes, including: cytokines, CD antigens, apoptosis markers, hormones, metabolites, and growth factors.

Simplify Your Multiomics Workflow with Zymo’s Co-Purification Kits

Genomes, epigenomes, transcriptomes, proteomes, and metabolomes. The list goes on…

In this rapidly evolving ‘omics’ era, researchers are generally becoming less enthusiastic about analyzing a genome, a transcriptome, or a proteome, etc., in isolation. More and more, researchers want to study multiple ‘omes’ in parallel, and one of the most popular combinations nowadays is genome and transcriptome analysis from the same sample.