In our last article we looked at the workflow for a typical immunohistochemistry (IHC) experiment. Regardless of the tissue type under investigation, the target biomolecules, and the detection method chosen, it is necessary to apply a method to extract and analyse the information held on the stained slides. The detection method used will influence image analysis to some extent i.e. light microscopy or fluorescence microscopy is used to visualise slides after chromogenic or fluorescence detection, respectively. With the right equipment, it is possible to scan entire slides for easier viewing of entire tissue sections, further analysis and to get high-quality images in digital format for use in publications and presentations.