Science Hub

Welcome to Nordic BioSite's Life Science Blog. Here, we will provide commentary on current events and trends in biological sciences, interesting stories about important scientists, technical tips and suggestions, and much more. Read on and learn something new.
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Multiplex Real-Time PCR for Beginners

This post was originally posted on June 3rd 2020.

In case the name doesn’t give it away, multiplex real-time PCR involves amplifying multiple DNA or RNA targets simultaneously in a single PCR reaction. This requires the presence of a specific pair of primers and a complementary DNA-binding probe for each target under study.

Restriction Enzymes 101

This post was originally posted on October 28th 2020.

Restriction enzymes or restriction endonucleases recognise short, specific DNA sequences known as restriction recognition sites, and make double-stranded cuts at or adjacent to these sites. These enzymes are naturally occurring in bacteria and archaea where they function as an ancient sequence-directed immune system, chopping up phage DNA at restriction sites in the genome of the invading phage.

Next Generation Sequencing 101 – Part 2

Welcome back to our next generation sequencing (NGS) 101 mini-series! In Part 1, we introduced the basics of NGS and provided an overview of some of its major applications. In this follow-up, we take a look at the typical NGS workflow including tips for good sample collection and NGS library preparation.

Next Generation Sequencing 101

Next generation sequencing, or simply NGS, has completely changed the way we do biological research. Also referred to as massively parallel sequencing, NGS offers high-throughput and high-resolution methods to decipher nucleic acid sequences, which provides important clues about protein function, regulatory and signalling pathways, mechanisms of human, plant and animal disease, diagnostics, and much more.

This blog post will introduce you to the basics of NGS and provide an overview of some of its major applications.

International Day of Immunology Is Here!

Today, we celebrate International Day of Immunology (DoI) – an annual day-long event dedicated to increasing global awareness of the importance of immunology in the fight against infectious diseases, autoimmunity and cancer.

Plex It up with LEGENDplex™ Multiplex Bead-Based Immunoassays!

Updated in April 2022.

LEGENDplex™ from BioLegend is a bead-based multiplex immunoassay that allows you to simultaneously detect and quantify up to 14 different analytes from panels that span hundreds of protein, chemokine, and cytokine targets across the fields of cardiovascular biology, extracellular matrix biology, immunology, metabolism and endocrinology, stem cell biology and toxicity.