Science Hub

Life Science Blog

Welcome to Nordic BioSite's Life Science Blog. Here, we will provide commentary on current events and trends in biological sciences, interesting stories about important scientists, technical tips and suggestions, and much more. Read on and learn something new.

RNA-Seq 3: Technology Overview

Welcome back to our RNA-seq article series! Now that we’ve gone through the general workflow and the ins and outs of RNA quality, we will look at some of the popular methods and platforms that you are likely to encounter if you embark on RNA-seq experiments.

Parkinson’s Disease & The Microbiome

Parkinson’s disease (PD) is a debilitating and incurable age-related neurodegenerative disease that affects bodily movements and worsens over time. It is one of the most common neurological diseases and its incidence is rising rapidly, with 14 million cases expected globally just 20 years from now.

World AIDs Day 2019

Yesterday was an important day for Christians all over the world. The first Advent candles were lit and millions of children started preparing their annual letters to Santa.

But did you know that yesterday was also World AIDS Day? In fact, it was the 31st AIDS day since the tradition started in 1988, when AIDSs was the first disease to get its own Global Health Day.

RNA-Seq 2: RNA Quality

Welcome back to our RNA-seq series! In Part 2, we take a look at RNA quality considerations for RNA-seq.

As outlined in Part 1, library preparation is a pivotal part of the RNA-seq workflow. Although the exact protocol will vary according to RNA-seq platform technology (more about this in Part 3), the overall goal is the same: to create a library of complementary DNA fragments that represent the originating RNA sample as accurately as possible and provide information about the RNA features of interest, including information about antisense transcripts, alternative splice variants, miRNAs and other non-coding RNAs, low-abundance transcripts and more.

Antibody Validation 101

Many researchers start their hunt for new antibodies by scouring the literature to find out which antibodies others are using for the same target. While this is a reasonable starting point, simply choosing an antibody with reported target specificity and sensitivity is not enough, even if the published data looks very convincing! In practice, the suitability of an antibody for a given sample type, experimental conditions, and application depends on many more parameters than affinity for a target protein.

RNA-Seq 1: Introduction

RNA sequencing or RNA-seq uses next-generation sequencing (NGS) technology to provide a snapshot of the numbers and identities of RNA molecules in any sample at any time under a condition(s) of interest. Since its inception in the early 00’s until now, data gleaned from RNA-seq experiments has revolutionised our understanding of RNA, its roles in animal and plant development, the importance of differential gene expression during health and disease, and how individuals respond to drug treatments. It has also revealed that eukaryotic transcriptomes are much more complex than previously thought!

Genotyping for Beginners

Genotyping encompasses the detection or mapping of small genetic differences between members of the same species and across different species. It is often used to gain insight into how genetic variation leads to phenotypic changes, including those that underpin the physical differences between individuals (e.g., human appearance) as well as those that manifest as disease. Other uses include epidemiology, forensics, plant breeding and ancient DNA analysis.

Beginner’s Guide to Immunohistochemistry 2: Choosing a Secondary Antibody

Welcome back to our beginner’s guide to immunohistochemistry (IHC). In this post, we’ll follow on from our last article on choosing a primary antibody, bringing you the most important considerations for choosing a secondary antibody.

Sample Collection and Preservation – Critical Starting Points in Your Research!

In the current ‘omics’ era, there seems to be no limit to the number of ways we can investigate our favorite organism or cell type. We can study genomes, transcriptomes, epigenomes, metabolomes, proteomes, kinomes, and more. Parallel advances in flow cytometry and other immunological techniques make it possible for us to simultaneously detect and profile new and characterized cell surface markers in robust multiplex setups.

Zombie Fixable Viability Dyes – When It’s a Matter of Life and Death

In any experiment, the quality of your data will be influenced to a large extent by the quality of the sample(s) used. Flow cytometry is no exception to that rule.

The Lowdown on Real-Time PCR – Part 2

Welcome to part 2 of our real-time PCR series. In part 1, we went through the basics of real-time PCR, its advantages over end-point PCR, a typical workflow, data output, and the choice of fluorescent labeling systems available.

In part 2, we take a look at the different quantification methods available, setup tips, primer design and quality control.

The Lowdown on Real-Time PCR – Part 1

In case the name doesn’t give it away, real-time PCR is a PCR application that monitors DNA amplification in real time. This means that amplification is monitored during the PCR reaction, and not at the end of the reaction as with end-point PCR, where PCR products are typically analyzed post-run on agarose gels.