Article No
246-MAHu/IgE/FITC
Application | FC, IF |
Article No | 246-MAHu/IgE/FITC |
Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
Clone | NI 307 |
Clone Type | monoclonal |
Conjugation | FITC |
Cross Reactivity | The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for crossreactivity with other species. |
Description | Mouse anti Human IgE (class specific), conjugated with FITC |
Supplier | Nordic - MUbio |
Format | Purified monoclonal mouse IgG1 kappa conjugated with fluorescein isothiocyanate, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. |
Isotype | IgG1 |
Notes | The reactivity of the antiserum is restricted to a isotype specific determinant of the IgE molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunofluorescence staining of cytoplasmic Ig. To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgE and cell membrane IgE. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200. These data should be interpreted as general recommendations only. |
Previous Article No | 246-MAHu/IgE/FITC |
Product Type | Antibodies Secondary |
Protocol | ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence. |
Research area | Immunology |
Size | 0.2 mg |
Source / Host | mouse |
Species Reactivity | human |
Storage | The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4ºC, not refrozen, and preferably used the same day. |
Substrate / Buffer | Purified monoclonal mouse IgG1 kappa conjugated with fluorescein isothiocyanate, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. |
Technical Specifications | Purified monoclonal mouse IgG1 kappa conjugated with fluorescein isothiocyanate, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water. |
Product Page Updated | 2024-01-31T10:35:40.347Z |