Article No
LS-C744141-1
Application | ELISA, ICC, IF, IHC |
Article No | LS-C744141-1 |
Country Availability | SE, FI, DK, NO, FO, GL |
Clone Type | polyclonal |
Conjugation | TRITC |
Description | Mouse IgA IgG1 IgG2a IgG2b IgG3 IgM antibody LS-C744141 is a TRITC-conjugated goat polyclonal antibody to mouse Mouse IgA IgG1 IgG2a IgG2b IgG3 IgM. Validated for ELISA, ICC, IF and IHC. |
Supplier | LifeSpan Biosciences |
Notes | Mouse IgA IgG1 IgG2a IgG2b IgG3 IgM antibody LS-C744141 is a TRITC-conjugated goat polyclonal antibody to mouse Mouse IgA IgG1 IgG2a IgG2b IgG3 IgM. Validated for ELISA, ICC, IF and IHC. |
Product Type | Antibodies Secondary |
Protocol | The reactivity of the antiserum is directed to the major isotypes of the mouse immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal mouse plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse origin in the middle layer of the indirect test procedure.This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
Shipping Information | RT |
Size | 1 ml |
Source / Host | goat |
Species Reactivity | mouse |
Storage | -20°C, 4°C |
Substrate / Buffer | PBS, pH 7.2 |
Technical Specifications | The reactivity of the antiserum is directed to the major isotypes of the mouse immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal mouse plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse origin in the middle layer of the indirect test procedure.This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
Product Page Updated | 2024-04-12T11:52:25.099Z |
Volume | 1 ml |