Article No
LS-C391059-20
NCBI Number | 7184 |
Application | FC, IF, IHC, WB |
Article No | LS-C391059-20 |
Country Availability | SE, FI, DK, NO, FO, GL |
Clone | 9G10.F8.2 |
Clone Type | monoclonal |
Concentration | 0.2 mg/ml |
Conjugation | Unconjugated |
Description | GRP94 antibody LS-C391059 is an unconjugated rat monoclonal antibody to GRP94 (HSP90B1 / GP96) from chicken. It is reactive with human, mouse, rat and other species. Validated for Flow, IF, IHC and WB. |
Recommended Dilution | FC (0.5 - 1 ug/10e6 cells), IF (0.5 - 1 ug/ml), IHC-P (0.5 - 1 ug/ml), WB (0.5 - 1 ug/ml) |
Supplier | LifeSpan Biosciences |
Entrez Gene ID | 7184 |
Gene Symbol | HSP90B1 |
Immunogen | Purified glucose regulated protein 94 from chicken oviducts. |
Isotype | IgG2a k |
Notes | GRP94 antibody LS-C391059 is an unconjugated rat monoclonal antibody to GRP94 (HSP90B1 / GP96) from chicken. It is reactive with human, mouse, rat and other species. Validated for Flow, IF, IHC and WB. |
Alias Names | HSP90B1, ECGP, Endoplasmin, gp96 homolog, gp96, GRP-94, GRP94, Tumor rejection antigen 1, TRA1 |
Product Type | Antibodies Primary |
Protocol | Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This mAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grps are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer. Optimal dilution of the GRP94 antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. |
Purification | Protein G Purified |
Shipping Information | RT |
Size | 20 µg |
Source / Host | rat |
Species Reactivity | chicken, cow/bovine, dog/canine, guinea pig, hamster, horse/equine, human, monkey, mouse, pig/porcine, rabbit, rat, sheep/ovine |
Storage | 4°C |
Substrate / Buffer | 0.01% BSA, 0.05% sodium azide |
Technical Specifications | Chicken HSP90B1 / GP96 / GRP94 |
Product Page Updated | 2024-04-12T09:20:06.597Z |