Article No
LS-C390415-100
Mouse Anti-Human IgM Secondary Antibody (clone IM260 + IM373 + ICO-30)
Human IgM antibody LS-C390415 is an unconjugated mouse monoclonal antibody. Validated for Flow, IF, IHC and WB.
Antibodies Secondary
Specifications
| Application | FC, IF, IHC, WB |
| Article No | LS-C390415-100 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone | IM260 + IM373 + ICO-30 |
| Clone Type | monoclonal |
| Concentration | 0.2 mg/ml |
| Conjugation | Unconjugated |
| Description | Human IgM antibody LS-C390415 is an unconjugated mouse monoclonal antibody. Validated for Flow, IF, IHC and WB. |
| Recommended Dilution | FC (0.5 - 1 ug/10e6 cells), IF (0.5 - 1 ug/ml), IHC-P (0.5 - 1 ug/ml), WB (0.5 - 1 ug/ml) |
| Supplier | LifeSpan Biosciences |
| Immunogen | Recombinant heavy chain of human IgM (IM260 IM373 & ICO-30). |
| Isotype | IgG1, IgG2b k |
| Notes | Human IgM antibody LS-C390415 is an unconjugated mouse monoclonal antibody. Validated for Flow, IF, IHC and WB. |
| Product Type | Antibodies Secondary |
| Protocol | Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Optimal dilution of the Anti-IgM antibody cocktail should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. |
| Purification | Protein G Purified |
| Shipping Information | RT |
| Size | 100 µg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | 4°C |
| Substrate / Buffer | 0.01% BSA, 0.05% sodium azide |
| Technical Specifications | Human IgM |
| Product Page Updated | 2024-04-12T09:20:06.597Z |
