Article No
LS-C390197-100
GPC3 / Glypican 3
Glypican 3 antibody LS-C390197 is an unconjugated mouse monoclonal antibody to human Glypican 3 (GPC3) (aa511-580). Validated for Flow, IF and IHC.
Antibodies Primary
Specifications
| NCBI Number | 2719 |
| Application | FC, IF, IHC |
| Article No | LS-C390197-100 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone | 1G12 |
| Clone Type | monoclonal |
| Concentration | 0.2 mg/ml |
| Conjugation | Unconjugated |
| Description | Glypican 3 antibody LS-C390197 is an unconjugated mouse monoclonal antibody to human Glypican 3 (GPC3) (aa511-580). Validated for Flow, IF and IHC. |
| Recommended Dilution | FC (0.5 - 1 ug/10e6 cells), IF (0.5 - 1 ug/ml), IHC-P (0.5 - 1 ug/ml) |
| Supplier | LifeSpan Biosciences |
| Entrez Gene ID | 2719 |
| Gene Symbol | GPC3 |
| Immunogen | Recombinant fragment containing amino acids 511-580 from the human protein. |
| Isotype | IgG1 k |
| Notes | Glypican 3 antibody LS-C390197 is an unconjugated mouse monoclonal antibody to human Glypican 3 (GPC3) (aa511-580). Validated for Flow, IF and IHC. |
| Alias Names | GPC3, DGSX, Glypican proteoglycan 3, Glypican 3, Glypican-3, GTR2-2, Intestinal protein OCI-5, SGB, SGBS1, SGBS, MXR7, OCI-5, OCI5, SDYS, Secreted glypican-3, Heparan sulphate proteoglycan |
| Product Type | Antibodies Primary |
| Protocol | Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilm's tumor. In patients with HCC, GPC3 is overexpressed in neoplastic liver tissue and elevated in serum, but is undetectable in normal liver, benign liver, and the serum of healthy donors. GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. In the context of testicular germ cell tumors, GPC3 expression is up regulated in certain histologic subtypes, specifically yolk sac tumors and choriocarcinoma. A high level of GPC3 expression is also found in some types of embryonal tumors, such as Wilm's tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100% in follicular carcinoma and 70% in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma. Optimal dilution of the Glypican-3 antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min. |
| Purification | Protein G Purified |
| Shipping Information | RT |
| Size | 100 µg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | 4°C |
| Substrate / Buffer | PBS, 0.1mg/ml BSA, 0.05% sodium azide |
| Technical Specifications | Human GPC3 / Glypican 3 |
| Product Page Updated | 2024-04-12T09:20:06.597Z |
