Article No
LS-B1995-50
Application | FC, IHC |
Article No | LS-B1995-50 |
Country Availability | SE, FI, DK, NO, FO, GL |
Clone | HI98 |
Clone Type | monoclonal |
Concentration | 0.5 mg/ml |
Conjugation | Unconjugated |
Description | CD15 antibody LS-B1995 is an unconjugated mouse monoclonal antibody to human CD15 (SSEA-1 / Lewis x). Validated for Flow and IHC. Tested on 20 paraffin-embedded human tissues. |
Recommended Dilution | IHC-P (1:50) |
Supplier | LifeSpan Biosciences |
Immunogen | Human PBMCs were used as immunogen. |
Isotype | IgM |
Notes | CD15 antibody LS-B1995 is an unconjugated mouse monoclonal antibody to human CD15 (SSEA-1 / Lewis x). Validated for Flow and IHC. Tested on 20 paraffin-embedded human tissues. |
Alias Names | , SSEA-1/Lewis X/CD15 |
Product Type | Antibodies Primary |
Protocol | Immunohistochemistry: LS-B1995 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1995 was determined to be 1:50. |
Purification | Affinity Purified |
Shipping Information | RT |
Size | 50 µg |
Source / Host | mouse |
Species Reactivity | human |
Storage | 4°C |
Substrate / Buffer | Phosphate-buffered solution, pH 7.2, 0.09% sodium azide. Sourced from tissue culture supernatant. |
Technical Specifications | Clone HI98 has been described to specifically react with 3-fucosyl-N-acetyllactosamine (3-FAL), also called X-hapten, SSEA-1, CD15, or Lewis X. |
Product Page Updated | 2024-04-12T06:35:05.844Z |