Article No
LS-B12855-250
GAPDH
GAPDH antibody LS-B12855 is an unconjugated mouse monoclonal antibody to GAPDH from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Antibodies Primary
Specifications
| NCBI Number | 2597 |
| Application | ICC, IF, IHC, WB |
| Article No | LS-B12855-250 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone | 1D4 |
| Clone Type | monoclonal |
| Conjugation | Unconjugated |
| Description | GAPDH antibody LS-B12855 is an unconjugated mouse monoclonal antibody to GAPDH from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. |
| Recommended Dilution | ICC (1:100), IF (1:100), IHC-P (1:50), WB (1:1000) |
| Supplier | LifeSpan Biosciences |
| Entrez Gene ID | 2597 |
| Gene Symbol | GAPDH |
| Immunogen | The immunogen used to raise this antibody was extensively purified pig GAPDH. |
| Isotype | IgM k |
| Notes | GAPDH antibody LS-B12855 is an unconjugated mouse monoclonal antibody to GAPDH from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. |
| Alias Names | GAPDH, A1 40 kd subunit, Activator 1 40 kd subunit, G3PD, GAPD, G3pdh, Rfc40, Rf-c 40 kd subunit |
| Product Type | Antibodies Primary |
| Protocol | The antibody solution can be used at dilutions of at least 1:100 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:1000 or lower. It is very useful as a loading control for quantitative western blotting. |
| Purification | Tissue culture supernatant |
| Shipping Information | RT |
| Size | 250 µl |
| Source / Host | mouse |
| Species Reactivity | cow/bovine, human, mouse, pig/porcine, rat |
| Storage | -20°C |
| Substrate / Buffer | Tissue Culture Supernatant, 10 mM Sodium Azide |
| Technical Specifications | Clone 1D4 recognizes GAPDH specifically both in western blots and in immunocytochemical experiments. On blots clone 1D4 reveals a prominent ~38kDa band, and on cells in tissue culture the antibody stains in a punctate cytoplasmic fashion. clone 1D4 is known to react with GAPDH from human, cow, pig, mouse, rat and other mammals, and also recognizes avian GAPDH. Since GAPDH is one of the most conserved proteins known, it is likely that the antibody is effective on other species also. |
| Product Page Updated | 2024-04-12T06:35:05.844Z |
| Volume | 250 µl |
