Article No
182-HM1103-500UG
CD200R3, Mouse, mAb Ba103 – 500 µg
CD200R3, Mouse, mAb Ba103 – 500 µg
Antibodies Primary
Specifications
| Application | FC, FS, IP, WB |
| Article No | 182-HM1103-500UG |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone | Ba103 |
| Clone Type | monoclonal |
| Conjugation | Unconjugated |
| Description | CD200R3, Mouse, mAb Ba103 – 500 µg |
| Supplier | Hycult biotechnology |
| Format | 0.5 mg 0.2 μm filtered protein G purified antibody solution in PBS with a concentration of at least 0.5 mg/ml. The endotoxin concentration is < 24 EU/mg, determined with HIT302 LAL Assay. |
| Immunogen | Mouse primary basophils; cell-enriched bone marrow cells |
| Isotype | IgG2b |
| Notes | The monoclonal antibody Ba103 recognizes mouse CD200 Receptor-like 3 (CD200R3), a protein of ~38 kDa or ~74 kDa (reduced or non-reduced condition, respectively). CD200R3 presumably exists as a disulfide-linked dimer on the cell surface, it is a single-pass type I membrane protein. The OX-2, or CD200 receptor (CD200R), belongs to the immunoglobulin superfamily. CD200R contains two Ig-like extracellular domains, one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. and mediates inhibitory signals in myeloid cells. Mice deficient for the ligand CD200 develop enhanced experimental allergic encephalomyelitis and collagen-induced arthritis. Unlike CD200R, the CD200R-like receptors function as activating receptors. They contain short cytoplasmic tails and a lysine residue in the transmembrane region and are likely to signal via adaptor proteins such as DAP12, DAP10, FcRg, or CD3z. CD200R3 is expressed in uterus and bone marrow-derived mast cells (at protein level). It is expressed in uterus, spleen, bone marrow-derived dendritic, basophil and mast cells. CD200R3 is also expressed in the lung of N. brasiliensis-infected mice. It is weakly expressed in brain, testis, lung and thymus. CD200R3 is expressed preferentially on cells of the myeloid lineage, including mast cells and basophils. Both cell types are involved in the host defense system against pathogens and in the development of allergic disorders. They function as independent essential initiators of allergic reactions. Basophils are the least common leukocytes in the peripheral blood accounting approximately 0.5 % of all leukocytes. In vivo depletion of basophils by using monoclonal antibody Ba103 proved the essential role of basophils in the development and maintenance of IgE-mediated chronic allergic inflammation. The activating CD200 receptors, like CD200R3, play an important role in IgE-independent mast cell and basophil activation. Monoclonal antibody Ba103 activates basophils ex vivo and elicits systemic anaphylaxis when administered in vivo. Cross-linking of CD200R3 on MC/9 cells by monoclonal antibody Ba103 induces degranulation. Furthermore, monoclonal antibody Ba103 is useful for depletion of basophils. |
| Alias Names | CD200 receptor-like 3, CD200RLb, cell surface glycoprotein OX-2 receptor 3 |
| Previous Article No | HM1103-FS, 182-HM1103-500UG, HM1103-500UG |
| Product Type | Antibodies Primary |
| Research area | Immunology |
| Shipping Information | Room Temperature |
| Size | 500 µg |
| Source / Host | rat |
| Species Reactivity | mouse |
| Storage | Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year. |
| Substrate / Buffer | Quantities >500 µg will be send without preservative and carrier free. Quantities |
| Product Page Updated | 2024-04-05T09:42:44.568Z |
