Article No
CY-9050V2
CircuLex Human DJ-1/PARK7 ELISA Kit Ver.2
CircuLex Human DJ-1/PARK7 ELISA Kit Ver.2
Assay & Detection
Specifications
| Application | ELISA |
| Article No | CY-9050V2 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Components | Microplate, 10X Wash Buffer, Dilution Buffer, Human DJ-1/PARK7 Standard, 20X HRP conjugated Detection Antibody, Conjugate Dilution Buffer, Substrate Reagent, Stop Solution |
| Description | CircuLex Human DJ-1/PARK7 ELISA Kit Ver.2 |
| Supplier | MBL |
| Additional Information | The CycLex Research Product CircuLex Human DJ-1/PARK7 ELISA kit Ver.2 is used for the quantitative and highly sensitive measurement of human DJ-1/PARK7 in serum, plasma, cell lysate and other biological samples. |
| Notes | DJ-1 (PARK7/CAP1/RS) was originally cloned as a putative oncogene capable of transforming NIH-3T3 cells in cooperation with H-ras (1), a protein expressed in sperm (2), and a regulator of RNA-protein interactions (3). DJ-1 has also been isolated as a gene associated with autosomal early-onset Parkinson's disease (PD) (4). Taken together, DJ-1 appears to be involved in diverse biological processes (5). First, several lines of evidence suggest that DJ-1 plays a role in the oxidative stress response (6,7). In cu1tured mammalian ce11s, DJ-1 quenches reactive oxygen species and is converted into a variant with a more acidic isoelectric point (6,8). Therefore, DJ-1 protects against reactive oxygen species-induced cell death, and its suppression with small interfering RNA (siRNA) sensitizes cells to such insults (7-10). Second, DJ-1 modulates transcription through interaction with DJ-1-binding protein (11) as well as with protein inhibitor of activated STAT (PIAS) (12). The latter modulates the activity of various transcription factors, Third, DJ-1 has been recognized as a regulatory subunit of an RNA-binding protein (13), Fourth, DJ-1, which is structurally related to the molecular chaperone Hsp31, may have chaperone activity itself, preventing heat-induced aggregation of substrate proteins, including alpha-synuclein (14). In addition, severa1 1ines of evidence suggest that DJ-1 plays a role in human tumorigenesis. First, breast cancer patients have elevated levels of circulating DJ-1 and anti-DJ-1 autoantibodies compared to healthy and non-breast cancer patients (15). Secondly, DJ-1 protein is increased in primary non-small cell lung carcinoma samples (16). Thirdly, treatment of cells from the human lung cancer cell line NCI-H157 with paclitaxel and MEK inhibitor U0126 leads to a decrease in DJ-1 protein expression (16). |
| Product Type | Assay & Detection |
| Range | 25 pg/mL to 1,600 pg/mL |
| Research area | Metabolism |
| Sensitivity | better than 8.4 pg/mL of sample. |
| Shipping Information | 4°C |
| Size | 96 Assays |
| Species Reactivity | human |
| Storage | 4°C |
| Technical Specifications | DJ-1 (PARK7/CAP1/RS) was originally cloned as a putative oncogene capable of transforming NIH-3T3 cells in cooperation with H-ras (1), a protein expressed in sperm (2), and a regulator of RNA-protein interactions (3). DJ-1 has also been isolated as a gene associated with autosomal early-onset Parkinson's disease (PD) (4). Taken together, DJ-1 appears to be involved in diverse biological processes (5). First, several lines of evidence suggest that DJ-1 plays a role in the oxidative stress response (6,7). In cu1tured mammalian ce11s, DJ-1 quenches reactive oxygen species and is converted into a variant with a more acidic isoelectric point (6,8). Therefore, DJ-1 protects against reactive oxygen species-induced cell death, and its suppression with small interfering RNA (siRNA) sensitizes cells to such insults (7-10). Second, DJ-1 modulates transcription through interaction with DJ-1-binding protein (11) as well as with protein inhibitor of activated STAT (PIAS) (12). The latter modulates the activity of various transcription factors, Third, DJ-1 has been recognized as a regulatory subunit of an RNA-binding protein (13), Fourth, DJ-1, which is structurally related to the molecular chaperone Hsp31, may have chaperone activity itself, preventing heat-induced aggregation of substrate proteins, including alpha-synuclein (14). In addition, severa1 1ines of evidence suggest that DJ-1 plays a role in human tumorigenesis. First, breast cancer patients have elevated levels of circulating DJ-1 and anti-DJ-1 autoantibodies compared to healthy and non-breast cancer patients (15). Secondly, DJ-1 protein is increased in primary non-small cell lung carcinoma samples (16). Thirdly, treatment of cells from the human lung cancer cell line NCI-H157 with paclitaxel and MEK inhibitor U0126 leads to a decrease in DJ-1 protein expression (16). |
| Product Page Updated | 2023-12-29T16:20:16.638Z |
