Article No
Biosite-D3020
Application | DNA Purification |
Article No | Biosite-D3020 |
Country Availability | SE, FI, DK, NO, FO, GL |
Description | Quick-DNA Microprep Kit |
Supplier | Zymo Research |
Notes | Quick-DNA™ MicroPrep (50 Preps) w/ Zymo-Spin™ IC Columns (Capped)|Rapid purification of DNA from cells and whole blood. |
Previous Article No | BioSite-D3020 |
Product Type | Sample Preparation |
Research area | Molecular Biology |
Shipping Information | RT |
Size | 50 Preps |
Storage | RT |
Technical Specifications | Compatibility: Unique lysis buffer system omits the need for Proteinase K digestion for biological fluids and cell culture samples. Up to 5 µg total DNA is eluted into ≥10 µl (6 µl minimum) DNA Elution Buffer or water. Human whole blood will typically yield 1.5-3.5 µg DNA per 50 µl blood sampled. Mammalian tissues already homogenized yield: 1-3 µg DNA per mg skeletal, heart, and brain tissues and 3-5 µg DNA per mg liver, kidney and lung tissues.DNA size limits: Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. Detergent tolerance: ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS. DNA purity: High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8 Equipment needed: microcentrifuge, vortex Sample sources: Whole blood, plasma, or serum from humans, mice, rats, etc. Also, cells from culture, buccal cells, as well as a variety of biological liquids are effectively processed using this kit. Tissue already digested with Proteinase K or mechanically homogenized can also be processed. |
Product Page Updated | 2024-04-08T09:14:54.133Z |