Article No
BT-C7JFF2-100
Specifications
| MW | ~ 60 kDa |
| Application | WB |
| Article No | BT-C7JFF2-100 |
| Biosite Brand | Biosite |
| Country Availability | all |
| Conjugation | Unconjugated |
| Description | Beclin-1 |
| Supplier | Nordic Biosite |
| Immunogen | Purified recombinant human Beclin 1 protein fragments expressed in E.coli. |
| Notes | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of proteins activated in response to nutrient deprivation and in neurodegenerative conditions. One of the proteins critical to this process is Beclin-1, the mammalian orthologue of the yeast autophagy protein Apg6/Vps30. Beclin-1 can complement defects in yeast autophagy caused by loss of Apg6 and can also stimulate autophagy when overexpressed in mammalian cells. Mammalian Beclin-1 was originally isolated in a yeast two-hybrid screen for Bcl-2 interacting proteins and has been shown to interact with Bcl-2 and Bcl-xL, but not with Bax or Bak. While Beclin-1 is generally ubiquitously expressed, research studies have shown it is monoallelically deleted in 40-75% of sporadic human breast and ovarian cancers. Beclin-1 is localized within cytoplasmic structures including the mitochondria, although overexpression of Beclin-1 reveals some nuclear staining and CRM1-dependent nuclear export. Investigators have demonstrated that Beclin-1-/- mice die early in embryogenesis and Beclin-1-/+ mice have a high incidence of spontaneous tumors. Stem cells from the null mice demonstrate an altered autophagic response, although responses to apoptosis appeared normal. Researchers have also found that overexpression of Beclin-1 in virally infected neurons in vivo resulted in significant protection against Sindbis virus-induced disease and neuronal apoptosis. |
| Alias Names | GT197; Beclin-1; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197 |
| Product Type | Antibodies Primary |
| Protocol | WB (1/500 - 1/1000) |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Size | 100ul |
| Source / Host | mouse |
| Species Reactivity | human, mouse, rat, monkey, hamster |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Substrate / Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Swiss-Prot No | Q14457 |
| Product Page Updated | 2024-02-19T11:09:15.120Z |
