Article No
BPS-80213
MMP2, His-tag (HEK293-derived) Recombinant
MMP2, His-tag (HEK293-derived) Recombinant
Proteins & Peptides
Specifications
| MW | 72 kDa |
| Accession Number | NM_004530 |
| Additional Information | Human matrix metallo-peptidase-2 (MMP2), also known as gelatinase A (GenBank Accession No. NM_004530), a.a. 30 - 660 (end), with C-terminal His-tag, MW=72 kDa, expressed in an HEK293 cell expression system. |
| Article No | BPS-80213 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Description | MMP2, His-tag (HEK293-derived) Recombinant |
| Supplier | BPS Bioscience |
| Entrez Gene ID | 4313 |
| Format | Aqueous buffer solution |
| Gene Symbol | MMP2 |
| Notes | Human matrix metallo-peptidase-2 (MMP2), also known as gelatinase A (GenBank Accession No. NM_004530), a.a. 30 - 660 (end), with C-terminal His-tag, MW=72 kDa, expressed in an HEK293 cell expression system. |
| Alias Names | matrix metallo-peptidase-2, MMP2, gelatinase A, MMP |
| Product Type | Proteins & Peptides |
| Protocol | Activation: This is a proform enzyme and_x000D_ requires activation prior to testing activity._x000D_ Dilute the enzyme to 100 ng/ml in a solution_x000D_ containing 50 mM HEPES, pH 7.4, 10 mM_x000D_ CaCl2, 0.05% Brij-35, and 1 mM APMA_x000D_ (amino-phenyl mercuric acetate). Incubate at_x000D_ 37ºC for 2 hours._x000D_ <br>Assay conditions: Reaction mixture with 10 µM_x000D_ 390 MMP substrate 1 (Anaspec). Incubate for_x000D_ 30 minutes at room temperature. Fluorescence_x000D_ intensity is measured at exc328/em393. |
| Shipping Information | -80°C (dry ice) |
| Size | 20 µg |
| Species Reactivity | human |
| Storage | At least 6 months at -80°C. |
| Substrate / Buffer | 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol |
| Technical Specifications | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| UniProt Number | P08253 |
| Product Page Updated | 2024-01-09T13:23:45.900Z |
