Article No
BPS-60612
AP1 Reporter Kit (JNK Pathway)
AP1 Reporter Kit (JNK Pathway)
Specifications
| Additional Information | The stress-activated protein kinase/c-jun N-terminal kinase (SAPK/JNK) family of proteins includes mitogen-activated protein kinases (MAPKs) that are activated by stress, inflammatory cytokines, mitogens, oncogenes, and inducers of cell differentiation and morphogenesis. Upon activation of the SAPK/JNK pathway, MAP Kinase Kinases phosphorylate and activate JNKs. The activated JNKs translocate to the nucleus where they phosphorylate and activate transcription factors such as c-Jun. The activated c-Jun forms homodimers or heterodimers with fos family proteins which bind to the activator protein-1(AP1) response element and induce target gene transcription. |
| Article No | BPS-60612 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Concentration | Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each) |
| Description | AP1 Reporter Kit (JNK Pathway) |
| Supplier | BPS Bioscience |
| Format | assay kit |
| Notes | The AP1 Reporter Kit is designed for monitoring the activity of the JNK signaling pathway and the transcriptional activity of AP1 in cultured cells. The kit contains a transfection-ready AP1 luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized AP1 responsive elements located upstream of a minimal promoter. The AP1 reporter is premixed with a constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity. |
| Alias Names | JNK reporter, SAPK reporter |
| Shipping Information | -20°C |
| Size | 500 rxns. |
| Storage | Stable at least 12 months from date of receipt, when stored as directed (-20°C) |
| Product Page Updated | 2024-04-26T09:56:27.777Z |
