Article No
BPS-51029
EZH2 (Y641C) /EED/SUZ12/RbAp48/AEBP2, His-tag, FLAG-tag Recombinant
EZH2 (Y641C) /EED/SUZ12/RbAp48/AEBP2, His-tag, FLAG-tag Recombinant
Proteins & Peptides
Specifications
| MW | 325 kDa (complex) |
| Additional Information | EZH2 is the catalytic subunit of the PRC2/EED-EZH2 complex, which methylates Lys9 (H3K9me) and Lys27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate Lys27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4. |
| Article No | BPS-51029 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Description | EZH2 (Y641C) /EED/SUZ12/RbAp48/AEBP2, His-tag, FLAG-tag Recombinant |
| Supplier | BPS Bioscience |
| Format | Aqueous buffer solution |
| Notes | Mutant version of our regular EZH2 5-member complex (Cat. #51004), but with a Tyr-to-Cys mutation on a.a. 646 of the EZH2 protein. Complex of human EZH2 (GenBank Accession No. NM_004456), a.a. 2-end; Y646C*, with N-terminal His tag, MW= 86 kDa, human EED (NM_003797), a.a. 2-end with N-terminal Flag tag, MW= 51 kDa, human SUZ12 (NM_015355), a.a. 2-end with N-terminal His tag, MW = 87 kDa, Human AEBP2 (NM_153207), a.a. 2-end with N-terminal His tag, MW= 53 kDa, and human RbAp48 (NM_005610), a.a. 2-end with N-terminal His tag, MW = 48 kDa, co-expressed in baculovirus expression system. |
| Alias Names | KTM6A, EZH2, PRC2, Enhancer Of Zeste Homolog 2, EZH2 (Y646C), EZH, Retinoblastoma Binding Protein 4 (RBBP4) |
| Product Type | Proteins & Peptides |
| Shipping Information | -80°C (dry ice) |
| Size | 20 µg |
| Source / Host | insect |
| Species Reactivity | human |
| Storage | At least 6 months at -80°C. |
| Substrate / Buffer | 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 10% glycerol, and 3 mM DTT. |
| UniProt Number | Q15910 |
| Product Page Updated | 2024-05-03T09:13:48.379Z |
