Article No
AG-CR1-3600-M005
JC-10 (high purity)
JC-10 (high purity)
Chemicals & Biochemicals
Specifications
| CAS No | n.d. |
| MW | ~600 |
| Additional Information | Application Protocols for JC-1: See literatures 1-3. |
| Article No | AG-CR1-3600-M005 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Description | JC-10 (high purity) |
| Supplier | Adipogen Life Sciences |
| Additional Information | Application Protocols for JC-1: See literatures 1-3. |
| Format | Powder |
| Notes | Chemical. MW: ~600. JC-10 is superior analog of JC-1, useful for determining mitochondrial membrane potential (MMP) in cells by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays. JC-10 is a cationic, lipophilic dye that accumulates in mitochondria of cells with a polarized mitochondrial membrane, selectively generating an orange J-aggregate emission profile (lambdaex = 540 nm/lambdaem = 590 nm) in healthy cells. Upon cell injury or cell death, as membrane potential decreases, which results in the failure to retain JC-10 aggregates in the mitochondria and JC-10 monomers are generated resulting in a shift to green emission (lambdaex = 490 nm/lambdaem = 525 nm). JC-10 shows improved solubility compared to JC-1 in aqueous media and an ability to detect subtler changes in mitochondrial membrane potential loss. JC-10 allows for qualitative visualization (shift from orange to green fluorescence) and quantitative detection (fluorescence intensity ratio) of mitochondrial membrane potential changes. Wavelength Maxima: lambdaex = 510 nm | lambdaem = 525 nm.|JC-10 is superior analog of JC-1, useful for determining mitochondrial membrane potential (MMP) in cells by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays. JC-10 is a cationic, lipophilic dye that accumulates in mitochondria of cells with a polarized mitochondrial membrane, selectively generating an orange J-aggregate emission profile (lambdaex = 540 nm/lambdaem = 590 nm) in healthy cells. Upon cell injury or cell death, as membrane potential decreases, which results in the failure to retain JC-10 aggregates in the mitochondria and JC-10 monomers are generated resulting in a shift to green emission (lambdaex = 490 nm/lambdaem = 525 nm). JC-10 shows improved solubility compared to JC-1 in aqueous media and an ability to detect subtler changes in mitochondrial membrane potential loss. JC-10 allows for qualitative visualization (shift from orange to green fluorescence) and quantitative detection (fluorescence intensity ratio) of mitochondrial membrane potential changes. Wavelength Maxima: lambdaex = 510 nm | lambdaem = 525 nm. |
| Alias Names | Enhanced JC-1 |
| Product Type | Chemicals & Biochemicals |
| Protocol | Application Protocols for JC-1: See literatures 1-3. |
| Purity | >98% |
| Research area | Metabolism |
| Shipping Information | RT |
| Size | 5 mg |
| Solubility | Soluble in water or DMSO. |
| Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Storage | -20°C, 4°C |
| Technical Specifications | Chemical. MW: ~600. JC-10 is superior analog of JC-1, useful for determining mitochondrial membrane potential (MMP) in cells by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays. JC-10 is a cationic, lipophilic dye that accumulates in mitochondria of cells with a polarized mitochondrial membrane, selectively generating an orange J-aggregate emission profile (lambdaex = 540 nm/lambdaem = 590 nm) in healthy cells. Upon cell injury or cell death, as membrane potential decreases, which results in the failure to retain JC-10 aggregates in the mitochondria and JC-10 monomers are generated resulting in a shift to green emission (lambdaex = 490 nm/lambdaem = 525 nm). JC-10 shows improved solubility compared to JC-1 in aqueous media and an ability to detect subtler changes in mitochondrial membrane potential loss. JC-10 allows for qualitative visualization (shift from orange to green fluorescence) and quantitative detection (fluorescence intensity ratio) of mitochondrial membrane potential changes. Wavelength Maxima: lambdaex = 510 nm | lambdaem = 525 nm. |
| Product Page Updated | 2024-02-01T08:25:01.492Z |
