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Anti-Hu CD24 PerCP-Cy™5.5

Antibodies Primary

Make a customized order

Article No

AFC-PP937V-100

Application

Species Reactivity

human

Size

100 tests

Clone

SN3

Source / Host

mouse

Article No

AFC-PP937V-100

Application

Species Reactivity

human

Size

100 tests

Clone

SN3

Source / Host

mouse

Specifications
Suggested protocols
References

Specifications

Application	FC
Article No	AFC-PP937V-100
Biosite Brand	Biosite Flow
Country Availability	all
Clone	SN3
Clone Type	monoclonal
Conjugation	PerCP/Cy5.5
Description	Anti-Hu CD24 PerCP-Cy™5.5
Supplier	Nordic Biosite
Entrez Gene ID	100133941
Format	PerCP-Cy5.5
Immunogen	Glycoproteins purified from human NALM-1 cell line.
Isotype	IgG1
Notes	CD24, also known as heat-stable antigen (HSA) or nectadorin, is a small mucin-like GPI-anchored extracellular membrane glycoprotein expressed on several cell types, including B cells. When B cells are activated and induced to further maturation, however, CD24 begins to disappear. CD24 seems to act as a gate-keeper for lipid rafts, thereby regulating the activity of integrins and other proteins such as the chemokine receptor CXCR4; it is also a ligand for P-selectin. CD24 triggering induces apoptosis of B cell precursors but not in mature resting B cells, where it instead inhibits their ability to proliferate in response to activation. CD24 expression is associated with invasiveness and poorer prognosis of carcinomas and is a marker of exosomes secreted into urine and amniotic fluid.
Alias Names	HSA, nectadorin, SCLC4
Product Type	Antibodies Primary
Protocol	Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Purification	Purified antibody is conjugated with activated tandem dye of activated Peridinin-Chlorophyll Protein-Cyanine 5.5 (PerCP-Cy™5.5) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Size	100 tests
Source / Host	mouse
Species Reactivity	human
Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Substrate / Buffer	Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Technical Specifications	The antibody SN3 reacts with CD24, a 35-45 kDa heavily glycosylated cell surface antigen. CD24 is expressed by granulocytes, B lymphocytes and by some activated T cells and T cell malignancies. It is not expressed on human thymocytes.
UniProt Number	P25063
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

Flow Cytometry

References

*Všianská P, Říhová L, Varmužová T, Suská R, Kryukov F, Mikulášová A, Kupská R, Penka M, Pour L, Adam Z, Hájek R: Analysis of B-cell subpopulations in monoclonal gammopathies. Clin Lymphoma Myeloma Leuk. 2015 Apr;15(4):e61-71., URL: http://www.ncbi.nlm.nih.gov/pubmed/25578543 ,*Maliar A, Servais C, Waks T, Chmielewski M, Lavy R, Altevogt P, Abken H, Eshhar Z: Redirected T cells that target pancreatic adenocarcinoma antigens eliminate tumors and metastases in mice. Gastroenterology. 2012 Nov;143(5):1375-1384, URL: http://www.ncbi.nlm.nih.gov/pubmed?term=maliar%20a%20servais%20c%20waks%20t ,*Fukukawa T, Matsuzaki H, Haruta Y, Hara H, Seon BK: New monoclonal antibodies SN3, SN3a, and SN3b directed to sialic acid of glycoprotein on human non-T leukemia cells. Exp Hematol. 1986 Oct;14(9):850-5., URL: https://pubmed.ncbi.nlm.nih.gov/3489635/,*Barcos M, Pollard C, Fukukawa T, Seon BK: Follicular mantle zone cell subpopulations detected by monoclonal antibody SN3. Hematol Oncol. 1986 Oct-Dec;4(4):251-9., URL: https://pubmed.ncbi.nlm.nih.gov/3493967/,*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)., URL: ,*Solvason N, Kearney JF. The human fetal omentum: a site of B cell generation. J Exp Med. 1992 Feb 1;175(2):397-404., URL: https://pubmed.ncbi.nlm.nih.gov/1370683/,*Fischer GF, Majdic O, Gadd S, Knapp W. Signal transduction in lymphocytic and myeloid cells via CD24, a new member of phosphoinositol-anchored membrane molecules. J Immunol. 1990 Jan 15;144(2):638-41., URL: https://pubmed.ncbi.nlm.nih.gov/2153173/,*Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989)., URL: ,*Sutherland DR, Illingworth A, Marinov I, Ortiz F, Andreasen J, Payne D, Wallace PK, Keeney M: ICCS/ESCCA consensus guidelines to detect GPI-deficient cells in paroxysmal nocturnal hemoglobinuria (PNH) and related disorders part 2 - reagent selection and assay optimization for high-sensitivity testing. Cytometry B Clin Cytom. 2018 Jan;94(1):23-48., URL: https://www.ncbi.nlm.nih.gov/pubmed/29236353,,*Runz S, Mierke CT, Joumaa S, Behrens J, Fabry B, Altevogt P: CD24 induces localization of beta1 integrin to lipid raft domains. Biochem Biophys Res Commun. 2008 Jan 4;365(1):35-41., URL: https://www.ncbi.nlm.nih.gov/pubmed/17980703?ordin,*Chou YY, Jeng YM, Lee TT, Hu FC, Kao HL, Lin WC, Lai PL, Hu RH, Yuan RH: Cytoplasmic CD24 expression is a novel prognostic factor in diffuse-type gastric adenocarcinoma. Ann Surg Oncol. 2007 Oct;14(10):2748-58., URL: https://www.ncbi.nlm.nih.gov/pubmed/17680316?ordin,*Keller S, Rupp C, Stoeck A, Runz S, Fogel M, Lugert S, Hager HD, Abdel-Bakky MS, Gutwein P, Altevogt P: CD24 is a marker of exosomes secreted into urine and amniotic fluid. Kidney Int. 2007 Nov;72(9):1095-102., URL: https://www.ncbi.nlm.nih.gov/pubmed/17700640?ordin,*Schabath H, Runz S, Joumaa S, Altevogt P: CD24 affects CXCR4 function in pre-B lymphocytes and breast carcinoma cells. J Cell Sci. 2006 Jan 15;119(Pt 2):314-25., URL: https://www.ncbi.nlm.nih.gov/pubmed/16390867?ordin,*Suzuki T, Kiyokawa N, Taguchi T, Sekino T, Katagiri YU, Fujimoto J: CD24 induces apoptosis in human B cells via the glycolipid-enriched membrane domains/rafts-mediated signaling system. J Immunol. 2001 May 1;166(9):5567-77., URL: https://www.ncbi.nlm.nih.gov/pubmed/11313396?ordin,,

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