Article No
AFC-KDSLOV-0.1
Specifications
| Application | WB, IP, FC |
| Article No | AFC-KDSLOV-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | LAT-01 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | Anti-LAT Purified |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 27040 |
| Format | Purified |
| Immunogen | Bacterially produced recombinant polypeptide corresponding to the entire cytoplasmic domain of human LAT. |
| Isotype | IgG1 |
| Keywords | Adaptor Proteins (Human), Adaptor Proteins |
| Notes | LAT (linker for activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation. |
| Alias Names | IMD52 |
| Previous Article No | AFC-4282-2, AFC-KDSLOV-0.1 |
| Product Type | Antibodies Primary |
| Protocol | Immunoprecipitation: Tyrosine-phosphorylated form of LAT is not optimally recognized. Flow cytometry: Intracellular staining. Tyrosine-phosphorylated form of LAT is not optimally recognized.Western blotting: Recommended dilution: 1-2 μg/ml. |
| Purification | Purified by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Research area | Immunology |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | Store at 2-8°C. Do not freeze. |
| Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The antibody LAT-01 reacts with an intracellular epitope of LAT, a 36-38 kDa transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. |
| UniProt Number | O43561 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
