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Mouse IgG2b Isotype Control PerCP-Cy™5.5

Antibodies Isotype Control

Make a customized order

Article No

AFC-F8XMEA-0.1

Application

Size

0.1 mg

Clone

MPC-11

Source / Host

mouse

Article No

AFC-F8XMEA-0.1

Application

Size

0.1 mg

Clone

MPC-11

Source / Host

mouse

Specifications
Suggested protocols
References

Specifications

Application	FC
Article No	AFC-F8XMEA-0.1
Biosite Brand	Biosite Flow
Country Availability	all
Clone	MPC-11
Clone Type	monoclonal
Concentration	0.1 mg/ml
Conjugation	PerCP/Cy5.5
Description	Mouse IgG2b Isotype Control PerCP-Cy™5.5
Supplier	Nordic Biosite
Format	PerCP-Cy5.5
Immunogen	KLH-coupled trinitrophenol
Isotype	IgG2b k
Product Type	Antibodies Isotype Control
Protocol	Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Purification	Purified antibody is conjugated with activated tandem dye of activated Peridinin-Chlorophyll Protein-Cyanine 5.5 (PerCP-Cy™5.5) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Size	0.1 mg
Source / Host	mouse
Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Substrate / Buffer	Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Technical Specifications	This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

Flow Cytometry

References

*Liang XS, Zhou Y, Li CZ, Wan MB: Natural course of chronic hepatitis B is characterized by changing patterns of programmed death type-1 of CD8-positive T cells. World J Gastroenterol. 2010 Feb 7;16(5):618-24., URL: http://www.ncbi.nlm.nih.gov/pubmed/20128032 ,*Dewals BG, Vanderplasschen A: Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues. Vet Res. 2011 Aug 22;42(1):95., URL: https://pubmed.ncbi.nlm.nih.gov/21859474/,*Gupta D, Wang Q, Vinson C, Dziarski R: Bacterial peptidoglycan induces CD14-dependent activation of transcription factors CREB/ATF and AP-1. J Biol Chem. 1999 May 14;274(20):14012-20., URL: http://www.ncbi.nlm.nih.gov/pubmed?term=gupta%20d%20vinson%20c ,*Smed-Sörensen A, Moll M, Cheng TY, Loré K, Norlin AC, Perbeck L, Moody DB, Spetz AL, Sandberg JK: IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa. Blood. 2008 May 15;111(10):5037-46., URL: http://www.ncbi.nlm.nih.gov/pubmed/18337560 ,*Im JS, Tapinos N, Chae GT, Illarionov PA, Besra GS, DeVries GH, Modlin RL, Sieling PA, Rambukkana A, Porcelli SA: Expression of CD1d molecules by human schwann cells and potential interactions with immunoregulatory invariant NK T cells. J Immunol. 2006 Oct 15;177(8):5226-35., URL: https://pubmed.ncbi.nlm.nih.gov/17015708/,,,

Shipping info

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