Article No
AFC-J359JV-0.1
Specifications
| Application | FC |
| Article No | AFC-J359JV-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | mN1A |
| Clone Type | monoclonal |
| Concentration | 0.1 mg/ml |
| Conjugation | PE |
| Description | Anti-Notch1 PE |
| Recommended Dilution | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 4851 |
| Format | PE |
| Immunogen | GST fusion protein containing cdc10-NCR region of mouse Notch1 |
| Isotype | IgG1 k |
| Keywords | Transcription Regulation & Nuclear Proteins (Rodent), Transcription Regulation & Nuclear Proteins (Human) |
| Notes | Notch 1 is a 270-300 kDa transmembrane heterodimeric protein with multiple extracellular growth factor-like repeats, and with an intracellular domain consisting of multiple different domain types. It serves as a receptor for membrane ligands, such as Delta 1, Jagged 1 (CD339), and Jagged 2, and regulates cell fate decisions. Upon ligand binding the transmembrane form of Notch 1 is repeatedly cleaved to provide approximately 120 kDa Notch intracellular fragment (NICD), which translocates to the nucleus and acts as a part of transcriptional complexes that alter differentiation, proliferation, and apoptosis. The highest level of Notch 1 expression is in brain, lung and thymus. |
| Alias Names | AOS5, TAN1, hN1, AOVD1 |
| Previous Article No | AFC-5261-2, AFC-J359JV-0.1 |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining. |
| Purification | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Research area | Cell Proliferation |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | mouse, human |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody mN1A recognizes intracellular domain of Notch 1 protein, mainly its activated form. The unprocessed Notch 1 protein is recognized with lower affinity. |
| UniProt Number | P46531 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
