Article No
AFC-0LM6G7-100
Specifications
| Application | FC |
| Article No | AFC-0LM6G7-100 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | LT10 |
| Clone Type | monoclonal |
| Conjugation | PE |
| Description | Anti-Hu CD10 PE |
| Recommended Dilution | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 4311 |
| Format | PE |
| Immunogen | mouse NALM-6 leukemia pre-B cell line (tissue/cell preparation) |
| Isotype | IgG1 |
| Keywords | CD and Related Antigens (Human) |
| Notes | CD10 (neutral endopeptidase – NEP, common acute lymphocytic leukemia antigen – CALLA, membrane metallo-endopeptidase – MME, enkefalinase) is a 100-kDa cell surface zinc metalloprotease, cleaving peptide bonds on the N-terminus of hydrophobic amino acids and inactivating multiple physiologically active peptids. CD10 is expressed on various normal cell types, including lymphoid precursor cells, germinal center B lymhocytes, and some epithelial cells, and its expression level serves as a marker for diagnostics of many carcinomas. CD10 is also a differentiation antigen for early B-lymphoid progenitors in the B-cell differentiation pathway and has a key role in regulation of growth, differentiation and signal transduction of many cellular systems. |
| Alias Names | CALLA, Neprilysin, Neutral endopeptidase, Enkephalinase, Atriopeptidase, MME |
| Previous Article No | AFC-5183-2 |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
| Purification | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Research area | Immunology |
| Size | 100 tests |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The antibody LT10 reacts with an extracellular epitope of CD10 (CALLA - Common acute lymphatic leukemia antigen), a 100 kDa type II integral membrane protein. |
| UniProt Number | P08473 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
