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Anti-Hu CD13 PE

Antibodies Primary

Make a customized order

Article No

AFC-B51HON-100

Application

Size

100 tests

Clone

WM15

Source / Host

mouse

Species Reactivity

human

monkey

Article No

AFC-B51HON-100

Application

Size

100 tests

Clone

WM15

Source / Host

mouse

Species Reactivity

human

monkey

Specifications
Suggested protocols
References

Specifications

Application	FC
Article No	AFC-B51HON-100
Biosite Brand	Biosite Flow
Country Availability	all
Clone	WM15
Clone Type	monoclonal
Conjugation	PE
Description	Anti-Hu CD13 PE
Recommended Dilution	The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Supplier	Nordic Biosite
Entrez Gene ID	290
Format	PE
Immunogen	Human AML cells
Isotype	IgG1
Keywords	CD Antigens & MHC (Veterinary), CD and Related Antigens (Human)
Notes	CD13 (aminopeptidase N, APN) is a 150 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. This metalloprotease preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 has also role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas.
Alias Names	Aminopeptidase N, APN, PEPN, ANPEP, gp150, LAP1
Previous Article No	AFC-5178-2
Product Type	Antibodies Primary
Protocol	Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Purification	Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Research area	Immunology
Size	100 tests
Source / Host	mouse
Species Reactivity	human, monkey
Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Substrate / Buffer	Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Technical Specifications	The antibody WM15 recognises an extracellular epitope of human CD13 cell surface glycoprotein, a 150 kDa molecule expressed on granulocytes, endothelial cells, epithelial cells and myeloid progenitors.
UniProt Number	P15144
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

Flow Cytometry

References

*Favaloro EJ, Browning T, Facey D: CD13 (GP150; aminopeptidase-N): predominant functional activity in blood is localized to plasma and is not cell-surface associated. Exp Hematol. 1993 Dec;21(13):1695-701. , URL: https://www.ncbi.nlm.nih.gov/pubmed/7902291?dopt=A,*McCormack E, Mujic M, Osdal T, Bruserud O, Gjertsen BT: Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia. Blood. 2013 Feb 14;121(7):e34-42. doi: 10.1182/blood-2012-05-429555., URL: https://www.ncbi.nlm.nih.gov/pubmed/23243270,*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)., URL: ,*Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989)., URL: ,*Leukocyte Typing III., McMichael A.J. et al. (Eds.), Oxford University Press (1987)., URL: ,*Bradstock KF, Favaloro EJ, Kabral A, Kerr A, Hughes WG, Musgrove E: Myeloid progenitor surface antigen identified by monoclonal antibody. Br J Haematol. 1985 Sep;61(1):11-20., URL: https://www.ncbi.nlm.nih.gov/pubmed/4052321?dopt=A,*Bradstock KF, Favaloro EJ, Kabral A, Kerr A, Hughes WG, Berndt MC, Musgrove E: Human myeloid differentiation antigens identified by monoclonal antibodies: expression on leukemic cells. Pathology. 1985 Jul;17(3):392-9. , URL: https://www.ncbi.nlm.nih.gov/pubmed/3865147?dopt=A,,*Tokuhara T, Hattori N, Ishida H, Hirai T, Higashiyama M, Kodama K, Miyake M. Clinical significance of aminopeptidase N in non-small cell lung cancer. Clin Cancer Res. 2006 Jul 1;12(13):3971-8., URL: https://pubmed.ncbi.nlm.nih.gov/16818694/,*Petrovic N, Schacke W, Gahagan JR, O'Conor CA, Winnicka B, Conway RE, Mina-Osorio P, Shapiro LH. CD13/APN regulates endothelial invasion and filopodia formation. Blood. 2007 Jul 1;110(1):142-50., URL: https://pubmed.ncbi.nlm.nih.gov/17363739/,*Terauchi M, Kajiyama H, Shibata K, Ino K, Nawa A, Mizutani S, Kikkawa F. Inhibition of APN/CD13 leads to suppressed progressive potential in ovarian carcinoma cells. BMC Cancer. 2007 Jul 27;7:140., URL: https://pubmed.ncbi.nlm.nih.gov/17655775/,,

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