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Anti-Hu CD54 PE

Antibodies Primary

Make a customized order

Article No

AFC-TMGYJC-100

Application

Size

100 tests

Clone

MEM-111

Source / Host

mouse

Species Reactivity

human

cow/bovine

rat

Article No

AFC-TMGYJC-100

Application

Size

100 tests

Clone

MEM-111

Source / Host

mouse

Species Reactivity

human

cow/bovine

rat

Specifications
Suggested protocols
References

Specifications

Application	FC
Article No	AFC-TMGYJC-100
Biosite Brand	Biosite Flow
Country Availability	all
Clone	MEM-111
Clone Type	monoclonal
Conjugation	PE
Description	Anti-Hu CD54 PE
Recommended Dilution	The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Supplier	Nordic Biosite
Entrez Gene ID	3383
Format	PE
Immunogen	Raji human Burkitt's lymphoma cell line
Isotype	IgG2a
Keywords	CD Antigens & MHC (Veterinary), CD and Related Antigens & MHC (Rodent), CD and Related Antigens (Human)
Notes	CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
Alias Names	ICAM-1, BB2, P3.58
Previous Article No	AFC-5130-2
Product Type	Antibodies Primary
Protocol	Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Purification	Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Research area	Immunology
Size	100 tests
Source / Host	mouse
Species Reactivity	human, cow/bovine, rat
Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Substrate / Buffer	Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Technical Specifications	The antibody MEM-111 reacts with an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation; it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.
UniProt Number	P05362
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

Flow Cytometry

References

*Nagaraju K et al.: A variety of cytokines and immunologically relevant surface molecules are expressed by normal human skeletal muscle cells under proinflammatory stimuli. Clin Exp Immunol 113, 407 (1998)., URL: https://www.ncbi.nlm.nih.gov/pubmed/9737670?dopt=A,*Tachimoto H. et al., Eotaxin-2 alters eosinophil integrin function via mitogen-activated protein kinases. Am J Respir Cell Mol Biol 26, 645 (2002)., URL: https://pubmed.ncbi.nlm.nih.gov/12034562/,*Burdick MM et al.: Colon carcinoma cell glycolipids, integrins, and other glycoproteins mediate adhesion to HUVECs under flow. Am J Physiol Cell Physiol 284, C977 (2003)., URL: https://www.physiology.org/doi/full/10.1152/ajpcel,*Hertel L et al.: Susceptibility of Immature and Mature Langerhans Cell-Type Dendritic Cells to Infection and Immunomodulation by Human Cytomegalovirus. J Virol. 77, 7563 (2003)., URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC16478,*Bacáková L, Mares V, Lysá V, Svorcík V: Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions. Biomaterials. 2000 Jun;21(11):1173-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/10817270?ordin,*Lee DJ, Sieling PA, Ochoa MT, Krutzik SR, Guo B, Hernandez M, Rea TH, Cheng G, Colonna M, Modlin RL: LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells. J Immunol. 2007 Dec 15;179(12):8128-36., URL: https://www.ncbi.nlm.nih.gov/pubmed/18056355,*Bacáková L, Mares V, Lysá V, Svorcík V: Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions. Biomaterials. 2000 Jun;21(11):1173-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/10602070?ordin,*Leeuwenberg JFM et al.: E-selectin and intercellular adhesion molecule-1 are released by activated human endothelial cells in vitro. Immunology 77, 543 (1992)., URL: https://www.ncbi.nlm.nih.gov/pubmed/1283598?dopt=A,*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)., URL: ,*Lee DJ, Sieling PA, Ochoa MT, Krutzik SR, Guo B, Hernandez M, Rea TH, Cheng G, Colonna M, Modlin RL: LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells. J Immunol. 2007 Dec 15;179(12):8128-36., URL: https://pubmed.ncbi.nlm.nih.gov/18056355/,,*Boyd AW, Wawryk SO, Burns GF, Fecondo JV: Intercellular adhesion molecule 1 (ICAM-1) has a central role in cell-cell contact-mediated immune mechanisms. Proc Natl Acad Sci U S A. 1988 May;85(9):3095-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/3362863?dopt=A,*Boyd AW, Dunn SM, Fecondo JV, Culvenor JG, Duhrsen U, Burns GF, Wawryk SO: Regulation of expression of a human intercellular adhesion molecule (ICAM-1) during lymphohematopoietic differentiation. Blood. 1989 May 15;73(7):1896-903., URL: https://www.ncbi.nlm.nih.gov/pubmed/2469503?dopt=A,*Springer TA: Adhesion receptors of the immune system. Nature. 1990 Aug 2;346(6283):425-34., URL: https://www.ncbi.nlm.nih.gov/pubmed/1974032?dopt=A,*Ockenhouse CF, Betageri R, Springer TA, Staunton DE: Plasmodium falciparum-infected erythrocytes bind ICAM-1 at a site distinct from LFA-1, Mac-1, and human rhinovirus. Cell. 1992 Jan 10;68(1):63-9. Erratum in: Cell 1992 Mar 6;68(5):following 994., URL: https://www.ncbi.nlm.nih.gov/pubmed/1346257?dopt=A,,

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