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Anti-Hu CD82 FITC

Antibodies Primary

Make a customized order

Article No

AFC-03YH75-100

Application

Species Reactivity

human

Size

100 tests

Clone

C33

Source / Host

mouse

Article No

AFC-03YH75-100

Application

Species Reactivity

human

Size

100 tests

Clone

C33

Source / Host

mouse

Specifications
Suggested protocols
References

Specifications

Application	FC
Article No	AFC-03YH75-100
Biosite Brand	Biosite Flow
Country Availability	all
Clone	C33
Clone Type	monoclonal
Conjugation	FITC
Description	Anti-Hu CD82 FITC
Recommended Dilution	The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
Supplier	Nordic Biosite
Entrez Gene ID	3732
Format	FITC
Immunogen	C91/PL (human HTLV-1+ T cell line)
Isotype	IgG2a
Keywords	CD and Related Antigens (Human)
Notes	CD82 (KAI1), a member of the tetraspanin family, forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules. These complexes influence adhesion, morphology, activation, proliferation and differentiation of B, T and other cells. CD82 regulates cytoskeleton rearrangement and may participate in the turnover of the tetraspanin complex members. Besides in the plasma membrane, CD82 is localized also in endosome/lysosome compartments. Tumour-suppressive roles of CD82 have been demonstrated.
Alias Names	R2, 4F9, C33, IA4, ST6, GR15, KAI1, SAR2, TSPAN27
Previous Article No	AFC-5025-2
Product Type	Antibodies Primary
Protocol	Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Purification	Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Research area	Immunology
Size	100 tests
Source / Host	mouse
Species Reactivity	human
Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Substrate / Buffer	Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Technical Specifications	The mouse monoclonal antibody C33 recognizes an extracellular/luminal epitope of CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.
UniProt Number	P27701
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

Flow Cytometry

References

*Schatzlmaier P, Supper V, Göschl L, Zwirzitz A, Eckerstorfer P, Ellmeier W, Huppa JB, Stockinger H: Rapid multiplex analysis of lipid raft components with single-cell resolution. Sci Signal. 2015 Sep 22;8(395):rs11, URL: http://www.ncbi.nlm.nih.gov/pubmed/26396269 ,*Ueda T, Ichikawa T, Tamaru J, Mikata A, Akakura K, Akimoto S, Imai T, Yoshie O, Shiraishi T, Yatani R, Ito H, Shimazaki J: Expression of the KAI1 protein in benign prostatic hyperplasia and prostate cancer. Am J Pathol. 1996 Nov;149(5):1435-40., URL: http://www.ncbi.nlm.nih.gov/pubmed/8909232?ordinal,*Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ: Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes. J Biol Chem. 1998 Aug 7;273(32):20121-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/9685355?ordina,*Imai T, Kakizaki M, Nishimura M, Yoshie O: Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82. J Immunol. 1995 Aug 1;155(3):1229-39., URL: https://www.ncbi.nlm.nih.gov/pubmed/7636191?ordina,*Imai T, Yoshie O: C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells. J Immunol. 1993 Dec 1;151(11):6470-81., URL: https://www.ncbi.nlm.nih.gov/pubmed/8245480?ordina,*Fukudome K, Furuse M, Imai T, Nishimura M, Takagi S, Hinuma Y, Yoshie O: Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen in HTLV-1-positive T cells. J Virol. 1992 Mar;66(3):1394-401., URL: https://www.ncbi.nlm.nih.gov/pubmed/1738199?ordina,,,

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