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Anti-Hu CD19 Biotin

Antibodies Primary

Make a customized order

Article No

AFC-U0MXUW-0.1

Species Reactivity

human

Size

0.1 mg

Clone

LT19

Source / Host

mouse

Application

FC, IP

Article No

AFC-U0MXUW-0.1

Species Reactivity

human

Size

0.1 mg

Clone

LT19

Source / Host

mouse

Application

FC, IP

Specifications
Suggested protocols
References

Specifications

Application	FC, IP
Article No	AFC-U0MXUW-0.1
Biosite Brand	Biosite Flow
Country Availability	all
Clone	LT19
Clone Type	monoclonal
Concentration	1 mg/ml
Conjugation	Biotin
Description	Anti-Hu CD19 Biotin
Recommended Dilution	The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Supplier	Nordic Biosite
Entrez Gene ID	930
Format	Biotin
Immunogen	Daudi human Burkitt lymphoma cell line
Isotype	IgG1
Keywords	CD and Related Antigens (Human)
Notes	CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
Alias Names	B4, Leu-12, CVID3
Previous Article No	AFC-4875-2, AFC-U0MXUW-0.1
Product Type	Antibodies Primary
Protocol	Flow cytometry: Recommended dilution: 3-5 μg/ml.
Purification	Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
Research area	Immunology
Size	0.1 mg
Source / Host	mouse
Species Reactivity	human
Storage	Store at 2-8°C. Do not freeze.
Substrate / Buffer	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Technical Specifications	The antibody LT19 reacts with an extracellular epitope of CD19 (B4), a 95 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells; it is lost on plasma cells.
UniProt Number	P15391
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

Flow Cytometry

Immunoprecipitation

References

*Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005 Nov 15;106(10):3567-74. Epub 2005 Jul 26., URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=16046525&query_hl=14&itool=pubmed_DocSum ,*Stehlíková O, Chovancová J, Tichý B, Krejčí M, Brychtová Y, Panovská A, Francová Skuhrová H, Burčková K, Borský M, Loja T, Mayer J, Pospíšilová S, Doubek M: Detecting minimal residual disease in patients with chronic lymphocytic leukemia using 8-color flow cytometry protocol in routine hematological practice. Int J Lab Hematol. 2013 Sep 13. doi: 10.1111/ijlh.12149., URL: http://www.ncbi.nlm.nih.gov/pubmed/24028768 ,*Kayserova J, Vcelakova J, Stechova K, Dudkova E, Hromadkova H, Sumnik Z, Kolouskova S, Spisek R, Sediva A: Decreased dendritic cell numbers but increased TLR9-mediated interferon-alpha production in first degree relatives of type 1 diabetes patients. Clin Immunol. 2014 Jul;153(1):49-55., URL: http://www.ncbi.nlm.nih.gov/pubmed/24709112 ,*Elias F, Flo J, Lopez RA, Zorzopulos J, Montaner A, Rodriguez JM: Strong cytosine-guanosine-independent immunostimulation in humans and other primates by synthetic oligodeoxynucleotides with PyNTTTTGT motifs. J Immunol. 2003 Oct 1;171(7):3697-704. , URL: https://www.ncbi.nlm.nih.gov/pubmed/14500668?dopt=,*Allen JS, Pang K, Skowera A, Ellis R, Rackham C, Lozanoska-Ochser B, Tree T, Leslie RD, Tremble JM, Dayan CM, Peakman M: Plasmacytoid dendritic cells are proportionally expanded at diagnosis of type 1 diabetes and enhance islet autoantigen presentation to T-cells through immune complex capture. Diabetes. 2009 Jan;58(1):138-45., URL: https://pubmed.ncbi.nlm.nih.gov/18835928/,,*Shi X, Xie C, Chang S, Zhou XJ, Tedder T, Mohan C: CD19 hyperexpression augments Sle1-induced humoral autoimmunity but not clinical nephritis. Arthritis Rheum. 2007 Sep;56(9):3057-69., URL: https://pubmed.ncbi.nlm.nih.gov/17763445/,*van Zelm MC, Reisli I, van der Burg M, Castaño D, van Noesel CJ, van Tol MJ, Woellner C, Grimbacher B, Patiño PJ, van Dongen JJ, Franco JL: An antibody-deficiency syndrome due to mutations in the CD19 gene. N Engl J Med. 2006 May 4;354(18):1901-12., URL: https://pubmed.ncbi.nlm.nih.gov/16672701/,*Inabe K, Kurosaki T: Tyrosine phosphorylation of B-cell adaptor for phosphoinositide 3-kinase is required for Akt activation in response to CD19 engagement. Blood. 2002 Jan 15;99(2):584-9., URL: https://pubmed.ncbi.nlm.nih.gov/11781242/,*Fujimoto M, Poe JC, Jansen PJ, Sato S, Tedder TF: CD19 amplifies B lymphocyte signal transduction by regulating Src-family protein tyrosine kinase activation. J Immunol. 1999 Jun 15;162(12):7088-94., URL: https://pubmed.ncbi.nlm.nih.gov/10358152/,,

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