Article No
AFC-8I1ER8-0.1
Specifications
| Application | FC |
| Article No | AFC-8I1ER8-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | 3G3 |
| Clone Type | monoclonal |
| Concentration | 0.5 mg/ml |
| Conjugation | APC |
| Description | Anti-FoxP3 APC |
| Recommended Dilution | The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 50943 |
| Format | APC |
| Immunogen | Full-length His-tagged recombinant murine FoxP3 |
| Isotype | IgG1 k |
| Keywords | Transcription Regulation & Nuclear Proteins (Rodent), Transcription Regulation & Nuclear Proteins (Human) |
| Notes | FoxP3 (forkhead box protein 3), a highly conserved forkhead/winged-helix transcription factor, plays a crucial role in maintaining immune homeostasis by governing the development and function of regulatory T cells. It is constitutively expressed at high level in CD25+ CD4+ Treg cells and at low level in a CD25- CD4+ Treg cell subset. Defects in gene encoding FoxP3 protein cause the scurfy phenotype in mice, and in human the IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. |
| Alias Names | Scurfin, JM2, AIID, IPEX, PIDX, XPID, DIETER |
| Previous Article No | AFC-4776-2, AFC-8I1ER8-0.1 |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: Recommended dilution: 1-2 μg/ml. Intracellular staining |
| Purification | Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Research area | Cell Proliferation |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | human, mouse |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody 3G3 recognizes N-terminal region of FoxP3, a 47-55 kDa transcription factor (intracellular antigen), which is the master regulator in the development and function of regulatory T cells. |
| UniProt Number | Q9BZS1 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
