Article No
AFC-OVPLZD-0.1
Anti-Hu CD45 Purified
Anti-Hu CD45 Purified
Antibodies Primary
Specifications
| Application | FC, IP, WB, IHC, ICC |
| Article No | AFC-OVPLZD-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | HI30 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | Anti-Hu CD45 Purified |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 5788 |
| Format | Purified |
| Immunogen | human peripheral blood leukocytes |
| Isotype | IgG1 k |
| Keywords | CD and Related Antigens (Human) |
| Notes | CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. |
| Alias Names | LCA, T200, LY5, B220, GP180, TPC |
| Previous Article No | AFC-4533-2, AFC-OVPLZD-0.1 |
| Product Type | Antibodies Primary |
| Protocol | Western blotting: Recommended dilution: 1 μg/ml; non-reducing conditions, band around 200-250 kDa; positive control: JURKAT human leukemia T-cell lysate, Kg-1a human leukemia cell lysate; non-reducing conditions, 6% separating gel. Flow cytometry: Recommended dilution: 1-4 µg/ml |
| Purification | Purified by protein-G affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Research area | Immunology |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | human, monkey |
| Storage | Store at 2-8°C. Do not freeze. |
| Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody HI30 recognizes an extracellular epitope on all isoforms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets. |
| UniProt Number | P08575 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
