Article No
AFC-HFXYTA-0.1
Anti-Clathrin Heavy Chain Purified
Anti-Clathrin Heavy Chain Purified
Antibodies Primary
Specifications
| Application | FC, IP, WB, ICC, ELISA |
| Article No | AFC-HFXYTA-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | BF-06 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | Anti-Clathrin Heavy Chain Purified |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 1213 |
| Format | Purified |
| Isotype | IgM |
| Keywords | Endocytosis (Veterinary), Endocytosis (Rodent), Endocytosis (Human) |
| Notes | Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results in membrane invagination. The basic oligomers are composed of three clathrin heavy chain (180 kDa) and three light chain (30 kDa) subunits and the process of polymerization is dynamically regulated by the light chains. Interaction of clathrin with the plasma membrane is mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Another proteins, such as endophilin, epsin and amphiphysin are involved in membrane invagination and clathrin rearrangements. Finally, dynamin functions at the fission stage of clathrin-mediated endocytosis. |
| Alias Names | CHC, CLHC, MRD56 |
| Previous Article No | AFC-4415-2, AFC-HFXYTA-0.1 |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: Recommended dilution: 2-5 μg/ml; positive control: human blood leukocytes. Intracellular staining. |
| Purification | Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods). |
| Purity | > 95% (by SDS-PAGE) |
| Research area | Cell Proliferation |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | rat, cow/bovine, mouse, human, pig/porcine |
| Storage | Store at 2-8°C. Do not freeze. |
| Substrate / Buffer | Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide |
| Technical Specifications | The antibody BF-06 recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa intracellular protein involved in receptor-mediated endocytosis. |
| UniProt Number | Q00610 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
