Article No
AFC-7JNLFG-0.1
Mouse IgG1 Isotype Control Purified
Mouse IgG1 Isotype Control Purified
Antibodies Primary
Specifications
| Application | FC, IP, IHC, ICC, WB, ELISA |
| Article No | AFC-7JNLFG-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | PPV-06 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | Mouse IgG1 Isotype Control Purified |
| Supplier | Nordic Biosite |
| Format | Purified |
| Isotype | IgG1 |
| Keywords | Immunoglobulin Isotype Controls |
| Notes | The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PPV-06, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished. |
| Previous Article No | AFC-4364-2, AFC-7JNLFG-0.1 |
| Product Type | Antibodies Primary |
| Protocol | Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background. |
| Purification | Purified by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Size | 0.1 mg |
| Source / Host | mouse |
| Storage | Store at 2-8°C. Do not freeze. |
| Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | This mouse IgG1 monoclonal antibody (clone PPV-06) reacts with undefined epitope on a plant pathogen. |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
