Article No
AFC-51C4UX-0.1
Anti-beta-Tubulin Purified
Anti-beta-Tubulin Purified
Antibodies Primary
Specifications
| Application | WB, IHC, ICC |
| Article No | AFC-51C4UX-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | TU-06 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | Anti-beta-Tubulin Purified |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 81027 |
| Format | Purified |
| Immunogen | Beta-subunits of porcine brain tubulin. |
| Isotype | IgM |
| Keywords | Cytoskeleton: Tubulin System (Veterinary), Cytoskeleton: Tubulin System (Rodent), Cytoskeleton: Tubulin System (Human), Cytoskeleton: Microtubular System |
| Notes | The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening – this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer. It is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. |
| Alias Names | TUBB |
| Previous Article No | AFC-4198-2, AFC-51C4UX-0.1 |
| Product Type | Antibodies Primary |
| Protocol | Immunocytochemistry: Recommended dilution: 2 μg/ml, fixed and permeabilized cells; positive control: 3T3 mouse embryonal fibroblast cell line. Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml, positive tissue: heart. Western blotting: Recommended dilution: 1-2 μg/ml. |
| Purification | Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods). |
| Purity | > 95% (by SDS-PAGE) |
| Research area | Cell Proliferation |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | plant, mouse, rat, pig/porcine, microorganism, chicken, human, fish |
| Storage | Store at 2-8°C. Do not freeze. |
| Substrate / Buffer | Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide |
| Technical Specifications | The antibody TU-06 recognizes an epitope (aa 81-95) on phylogenetically conserved N-terminal structural domain of beta-tubulin (recognizes all beta-tubulin isoforms) in various species. |
| UniProt Number | Q9H4B7 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
