Article No
AFC-0AN285-0.1
Anti-Hu CD25 Purified
Anti-Hu CD25 Purified
Antibodies Primary
Specifications
| Application | FC |
| Article No | AFC-0AN285-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | MEM-181 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | Anti-Hu CD25 Purified |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 3559 |
| Format | Purified |
| Immunogen | PHA-activated peripheral blood leucocytes |
| Isotype | IgG1 |
| Keywords | CD and Related Antigens (Human) |
| Notes | CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas. |
| Alias Names | IL-2R α chain, IL-2-RA, IL2-RA, p55, TAC antigen, IDDM10, TCGFR |
| Previous Article No | AFC-4167-2, AFC-0AN285-0.1 |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: Recommended dilution: 2 μg/ml. |
| Purification | Purified by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Research area | Immunology |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | Store at 2-8°C. Do not freeze. |
| Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The antibody MEM-181 reacts with an extracellular epitope of CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes. |
| UniProt Number | P01589 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
