Article No
AFC-3C1HS8-0.1
Anti-Hu CD4 Purified
Anti-Hu CD4 Purified
Antibodies Primary
Specifications
| Application | FC, IP |
| Article No | AFC-3C1HS8-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | MEM-115 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | Anti-Hu CD4 Purified |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 920 |
| Format | Purified |
| Immunogen | Human thymocytes and T lymphocytes. |
| Isotype | IgG2a |
| Keywords | CD and Related Antigens (Human) |
| Notes | CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency). |
| Alias Names | T4/Leu-3, L3T4 |
| Previous Article No | AFC-4153-2, AFC-3C1HS8-0.1 |
| Product Type | Antibodies Primary |
| Protocol | Immunoprecipitation: Excellent. Flow cytometry: Recommended dilution: 3 μg/ml. Although it has not been tested rigorously, following data suggest that the antibody MEM-115 is a low-affinity antibody: its binding to T cells increases at elevated temperature, monovalent Fab fragments essentially do not bind to T cells. |
| Purification | Purified by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Research area | Immunology |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | Store at 2-8°C. Do not freeze. |
| Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The antibody MEM-115 recognizes an extracellular epitope in the D1 domain of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (“helper“ T cells) and also on monocytes, tissue macrophages and granulocytes. It is negative in Western blotting even with non-reduced samples of cell lysates. |
| UniProt Number | P01730 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
