Article No
AFC-G7OLFW-0.1
Application | FC, IP, WB, IHC |
Article No | AFC-G7OLFW-0.1 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | QBEnd-10 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | Anti-Hu CD34 Purified Azide Free |
Supplier | Nordic Biosite |
Entrez Gene ID | 947 |
Format | Purified Azide Free |
Immunogen | Human endothelial vesicles |
Isotype | IgG1 |
Keywords | CD and Related Antigens (Human) |
Notes | CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. |
Alias Names | Mucosialin |
Previous Article No | AFC-4109-2, AFC-G7OLFW-0.1 |
Product Type | Antibodies Primary |
Protocol | Functional application: The antibody QBEnd-10 induces homotypic adhesion of leukemic cell line. Flow cytometry: Recommended dilution: 5 μg/ml. Immunohistochemistry (paraffin sections): Recommended dilution: 2-8 μg/ml.Western blotting: Recommended dilution: 1-2 μg/ml, positive control: TF-1 cells. |
Purification | Purified by protein-A affinity chromatography. |
Purity | > 95% (by SDS-PAGE) |
Research area | Immunology |
Size | 0.1 mg |
Source / Host | mouse |
Species Reactivity | monkey, human |
Storage | Store at 2-8°C. Do not freeze. |
Substrate / Buffer | Phosphate buffered saline (PBS), pH 7.4 |
Technical Specifications | The antibody QBEnd-10 reacts with an extracellular class II epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. This antibody has been also used as an endothelial marker. |
UniProt Number | P28906 |
Product Page Updated | 2024-01-03T12:18:20.318Z |