Article No
AFC-KNS2IE-0.1
Specifications
| Application | FC |
| Article No | AFC-KNS2IE-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | EM-50 |
| Clone Type | monoclonal |
| Concentration | 0.1 mg/ml |
| Conjugation | PE |
| Description | Anti-R-RAS2 PE |
| Recommended Dilution | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 22800 |
| Format | PE |
| Immunogen | Human TC21 |
| Isotype | IgG1 k |
| Keywords | Signaling of Small GTPases (Human) |
| Notes | R-Ras2 / TC21 is the only member of R-Ras family of small GTPases that shows transforming activities similar to H-Ras, N-Ras, and K-Ras, and it is also structurally similar to them. R-Ras2 seems to play an important role in activating signal transduction pathways that control cell proliferation. Its mutations are associated with the growth of certain tumors, but also overexpression of the wild type form of R-Ras2 has been frequently detected in various carcinomas. Pseudogenes of R-Ras2 gene are found on chromosomes 1 and 2. Alternate splicing results in multiple transcript variants. |
| Alias Names | TC21, RRAS2 |
| Previous Article No | AFC-31E327, AFC-KNS2IE-0.1 |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining. |
| Purification | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody EM-50 recognizes R-Ras2 / TC21 protein (intracellular antigen) and does not react with R-Ras1, H-Ras, K-Ras, and N-Ras. |
| UniProt Number | P62070 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
