Article No
805-587-008
Application | Labeling |
Article No | 805-587-008 |
Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
Clone Type | polyclonal |
Conjugation | Alexa Fluor 594 |
Description | Alexa Fluor® 594-conjugated AffiniPure Fab Fragment Bovine Anti-Goat IgG, Fc Fragment Specific |
Recommended Dilution | Lbl 1:100 - 1:800 |
Supplier | Jackson ImmunoResearch |
Format | Affinity Purified |
Immunogen | IgG, Fc fragment |
Isotype | IgG |
Notes | Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies are monovalent, containing only a single antigen binding site. The molecular weight of Fab fragments is about 50 kDa. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of goat IgG heavy chain but not with the Fab portion of goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species. |
Product Type | Antibodies Secondary |
Protocol | 1:100 - 1:800 for sequential labeling applications. To complex with primary antibody in solution, use 1:1 weight ratio of Fab:primary antibody (3:1 molar ratio). Vortex and incubate for 30 minutes at room temperature prior to use. Titrate complex to optimal dilution for assay. Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically. |
Purity | AffiniPure |
Shipping Information | RT |
Size | 0.5 mg |
Source / Host | cow/bovine |
Species Reactivity | goat |
Storage | 4°C |
Substrate / Buffer | 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 |
Product Page Updated | 2023-12-29T14:15:50.354Z |