Article No
36-3723-20
Actin, Muscle Specific (Muscle Cell Marker)
Anti-Actin, Muscle Specific (Muscle Cell Marker) Monoclonal Antibody(Clone: HHF35)
Antibodies Primary
Specifications
| Application | FC, IF, IHC |
| Article No | 36-3723-20 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone | HHF35 |
| Clone Type | monoclonal |
| Conjugation | Unconjugated |
| Description | Anti-Actin, Muscle Specific (Muscle Cell Marker) Monoclonal Antibody(Clone: HHF35) |
| Supplier | Abeomics |
| Immunogen | SDS extract of human myocardium. |
| Isotype | IgG1 k |
| Notes | This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas). |
| Alias Names | ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1 |
| Product Type | Antibodies Primary |
| Protocol | Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) |
| Shipping Information | Blue Ice |
| Size | 20 µg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | 4°C, -80°C |
| Substrate / Buffer | 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| UniProt Number | P62737 |
| Product Page Updated | 2024-05-08T07:23:11.080Z |
