Article No
36-3289-100
p53 Tumor Suppressor Protein
Anti-p53 Tumor Suppressor Protein Monoclonal Antibody(Clone: BP53-12)
Antibodies Primary
Specifications
| Application | IHC, WB |
| Article No | 36-3289-100 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone | BP53-12 |
| Clone Type | monoclonal |
| Conjugation | Unconjugated |
| Description | Anti-p53 Tumor Suppressor Protein Monoclonal Antibody(Clone: BP53-12) |
| Supplier | Abeomics |
| Entrez Gene ID | 7157 |
| Gene Symbol | TP53 |
| Immunogen | Recombinant human wild-type p53 protein |
| Isotype | IgG2a k |
| Notes | This MAb reacts with an N-terminal epitope (aa 16-25) of both wild type and mutated p53. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. |
| Alias Names | Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53), Tumor Protein p53, Tumor Suppressor p53 |
| Product Type | Antibodies Primary |
| Protocol | Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) |
| Shipping Information | Blue Ice |
| Size | 100 µg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | 4°C, -80°C |
| Substrate / Buffer | 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| UniProt Number | P04637 |
| Product Page Updated | 2024-02-06T07:35:53.072Z |
