Article No
356-MB-019-4000
Article No | 356-MB-019-4000 |
Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
Concentration | 10X |
Conjugation | Unconjugated |
Description | 10X Tris-Borate-EDTA TBE Buffer |
Supplier | Rockland |
Format | Liquid (sterile filtered) |
Notes | TBE is often used in procedures involving nucleic acids, the most common being electrophoresis in molecular biology. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. EDTA is a chelator of divalent cations, particularly of magnesium (Mg2+). As these ions are necessary co-factors for many enzymes, including contaminant nucleases, the role of the EDTA is to protect the nucleic acids against enzymatic degradation. But since Mg2+ is also a co-factor for many useful DNA-modifying enzymes such as restriction enzymes and DNA polymerases. |
Alias Names | buffer containing boric acid and EDTA |
Product Type | Buffers & Mixes |
Shipping Information | Ambient |
Size | 4 x 1 L |
Storage | Store container at room temperature (18° to 26° C) prior to opening. |
Substrate / Buffer | See application note. |
Product Page Updated | 2024-03-07T15:25:27.010Z |