Article No
333-A8751-25mg
Specifications
| MW | 727.68 |
| Article No | 333-A8751-25mg |
| Country Availability | SE, FI, DK, NO, IS, EE, FO, GL |
| Description | Ac-VEID-AFC |
| Supplier | APExBIO |
| Notes | Ac-VEID-AFC is a fluorogenic substrate of caspase-6 (Km=30µM; Kcat/Km=168000 M-1sec-1) and related cysteine proteases. The sequence is based on the lamin A site of cleavage. During apoptosis, caspase-6 cleaves the substrate and release AFC which then can be quantified by fluorescent detection at Ex/Em = 400/505. Ac-IETD-pNA can be used to identify and quantify caspase-6 activity in apoptotic cell lysates and to study events downstream of caspase-6 activation. References:1. A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis: N.A. Thornberry, et al.; J. Biol. Chem. 272, 17907 (1997)2. Substrate specificities of caspase family proteases: R.V. Talanian, et al.; J. Biol. Chem. 272, 9677 (1997)3. The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32: S.M. Srinivasula, et al.; J. Biol. Chem. 271, 27099 (1996) |
| Molecular Formula | C32H40F3N5O11 |
| Previous Article No | 333-A8751-25MG |
| Product Type | Assay & Detection |
| Purity | 98% |
| Shipping Information | BLUE ICE |
| Size | 25 mg |
| Solubility | ≥72.8 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O |
| Storage | -20°C |
| Product Page Updated | 2024-03-05T10:50:49.934Z |