Article No
303-MUB2056P
Mouse anti CD11a, Integrin alpha-L
Mouse anti CD11a, Integrin alpha-L
Antibodies Primary
Specifications
| Application | WB, IP |
| Article No | 303-MUB2056P |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone | NKI (SPV)-L1 |
| Clone Type | monoclonal |
| Concentration | See vial for concentration |
| Conjugation | Unconjugated |
| Description | Mouse anti CD11a, Integrin alpha-L |
| Supplier | Exalpha Biologicals |
| Immunogen | A recombinant protein corresponding to amino acids 387 to 982 of the human retinoblastoma sequence |
| Isotype | IgG1 |
| Notes | Integrin alpha L (antigen CD11A, p180, lymphocyte function-associated antigen 1; alpha polypeptide), also known as ITGAL, is a human gene which functions in the immune system. It is involved in cellular adhesion and co-stimulatory signaling. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 is part of the family of leukocyte integrins that are recognised by their common β-chains (CD18) and the distinct α-chain CD11a. LFA-1 is expressed on lymphocytes, monocytes and granulocytes. It is involved in recruitment of these cells to the site of infection where it binds to ICAM-1 on antigen-presenting cells and functions as an adhesion molecule. LFA-1 is the first to bind T-cells to antigen-presenting cells and initially binds weakly. A signal from the T-cell receptor and/or the cytokine receptor changes the conformation and prolongs the cell contact, allowing the T-cell to proliferate. Blocking experiments with anti-LFA monoclonal antibodies demonstrated that LFA-1 inhibits the adhesion step between effector and target cells in cytotoxic T lymphocyte, natural killer and lectin dependent cellular cytotoxicity. LFA-1 also acts as an adhesion molecule between monocytes and T-cells. |
| Product Type | Antibodies Primary |
| Protocol | The NKI (SPV)-L1 antibody is suitable for the detection of CD11a by immunoprecipitation, immunocytochemistry, immunohistochemistry on frozen sections and by flow cytometry. Human tonsil is suitable for use as a positive control tissue. Optimal antibody dilutions for the different applications should be determined by titration. |
| Purification | Protein A/G Chromatography |
| Research area | Immunology |
| Shipping Information | Room Temperature |
| Size | 0.1mg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product. |
| Substrate / Buffer | Each vial contains 100µl 1mg/ml purified monoclonal antibody in phosphate buffered saline (PBS) containing 0.09% sodium azide. |
| Technical Specifications | The NKI (SPV)-L1 antibody is suitable for the detection of CD11a by immunoprecipitation, immunocytochemistry, immunohistochemistry on frozen sections and by flow cytometry. Human tonsil is suitable for use as a positive control tissue. Optimal antibody dilutions for the different applications should be determined by titration. |
| UniProt Number | UniProt: P20701 (Human) |
| Product Page Updated | 2023-12-29T14:25:47.027Z |
