Article No
303-ABaLFA-F
Chicken anti Bacillus anthracis Lethal Factor A, conjugated with FITC
Chicken anti Bacillus anthracis Lethal Factor A, conjugated with FITC
Antibodies Primary
Specifications
| Application | Blotting, ELISA, ICC, IHC |
| Article No | 303-ABaLFA-F |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone Type | polyclonal |
| Concentration | See vial for concentration. |
| Conjugation | FITC |
| Description | Chicken anti Bacillus anthracis Lethal Factor A, conjugated with FITC |
| Supplier | Exalpha Biologicals |
| Format | Liquid |
| Notes | Over the past decade our double-stranded RNA (dsRNA)antibodies have been used extensively to detect and characterise plant and animal viruses with dsRNA genomes or intermediates. In addition, the anti-dsRNA antibodies can be used as a diagnostic tool to detect pathogens, including detection in paraffin-embedded fixed tissue samples (Richardson et al. 2010). The J5 IgG2b antibody recognizes dsRNA with very similar affinity and specificity to our J2 antibody (see Schonborn et al., 1991), but has a different isotype – thus allowing more flexibility for the simultaneous detection of dsRNA with other markers, particularly in immunofluorescence microscopy, and has been used to detect replicative intermediates of the fish virus Infectious Pancreatic Necrosis Virus (IPNV) (Levican-Asenjo et al., 2019) or of ECMV in Vero cells. The J5 antibody can detect all tested forms of dsRNA, including poly(A):poly(U), poly(I):poly(C) and dsRNA from viruses such as Dengue Virus, Encephalomyocarditis Virus, Vaccinia Virus, Reovirus or Cucumber Mosaic Virus. Similarly to our other antibodies dsRNA-binding of J5 is sequence-independent, as long as the length of the dsRNA exceeds 40nt. The antibody does not react with ssRNA, ssDNA or dsDNA. J5 has been tested successfully in nucleic acid ELISA, immunoblotting and immunofluorescence microscopy. |
| Product Type | Antibodies Primary |
| Protocol | Optimal conjugate working dilutions should be determined for your particular assay conditions. |
| Purification | Egg-yolk derived IgY Affinity Purified |
| Purity | Gel electrophoretically pure IgG antibody. |
| Shipping Information | Ship at ambient temperature. |
| Size | 0.1 mg |
| Source / Host | chicken |
| Storage | Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles. |
| Substrate / Buffer | Phosphate Buffered Saline‚ 50% Glycerol with 0.075% sodium azide |
| Technical Specifications | Optimal conjugate working dilutions should be determined for your particular assay conditions. |
| Product Page Updated | 2023-12-29T14:25:47.027Z |
