Article No
246-SR/GAHu/BJL(SD+HD)/PO
Application | WB, ELISA |
Article No | 246-SR/GAHu/BJL(SD+HD)/PO |
Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
Clone | Polyclonal |
Clone Type | polyclonal |
Conjugation | HRP |
Cross Reactivity | Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail. |
Description | Goat anti Human Ig lambda light chains (free and bound), conjugated with Horseradish peroxidase |
Supplier | Nordic - MUbio |
Format | Horseradish peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. |
Notes | The reactivity of the antiserum is directed to the surface and hidden determinants of Ig lambda chain. A reaction is obtained with intact polyclonal or monoclonal immunoglobulins of the lambda type, as well as free lambda light chains. To identify light chain type in enzyme-immunocytochemical and immunohistochemical staining of immunoglobulins and to demonstrate circulating monoclonal immunoglobulins and Bence Jones proteins of the lambda type in serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
Previous Article No | 246-SR/GAHu/BJL(SD+HD)/PO |
Product Type | Antibodies Secondary |
Protocol | ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting. |
Research area | Immunology |
Size | 1 ml |
Source / Host | goat |
Species Reactivity | human |
Storage | The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. |
Substrate / Buffer | Horseradish peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. |
Technical Specifications | Horseradish peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. |
Product Page Updated | 2024-01-31T10:35:40.347Z |