Article No
246-MUB1102P
Mouse anti Lamin A and C
Mouse anti Lamin A and C
Antibodies Primary
Specifications
| Application | FC, ICC, IHC, WB |
| Article No | 246-MUB1102P |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone | 131C3 |
| Clone Type | monoclonal |
| Description | Mouse anti Lamin A and C |
| Supplier | Nordic - MUbio |
| Format | Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide. |
| Isotype | IgG1 |
| Notes | Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins. |
| Previous Article No | 246-MUB1102P |
| Product Type | Antibodies Primary |
| Protocol | 131C3 is suitable for immunocytochemistry, immunohistochemistry on frozen sections, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 – 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications. |
| Research area | Cell Proliferation |
| Shipping Information | Ship at ambient temperature. |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | cow/bovine, dog/canine, hamster, human, mouse, rat, sheep/ovine |
| Storage | The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product. |
| Substrate / Buffer | Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide. |
| Technical Specifications | Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide. |
| UniProt Number | P02545 |
| Product Page Updated | 2024-01-31T10:35:40.347Z |
