Article No
182-HK317-02
HNP1-3, Human, ELISA kit – 2 x 96 det.
HNP1-3, Human, ELISA kit – 2 x 96 det.
Assay & Detection
Specifications
| Application | ELISA |
| Article No | 182-HK317-02 |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Description | HNP1-3, Human, ELISA kit – 2 x 96 det. |
| Supplier | Hycult biotechnology |
| Additional Information | Human neutrophil defensins (alpha-defensins, HNP1-3) belong to the family of cationic trisulfide-containing microbicidal peptides. |
| Keywords | Autoimmunity , Infectious diseases |
| Notes | Human neutrophil defensins (alpha-defensins, HNP1-3) belong to the family of cationic trisulfide-containing microbicidal peptides. Besides microbicidal, the peptides exert chemotactic, immunomodulating and cytotoxic activity and participate in host defense and inflammation. Azurophilic granules of neutrophils contain Human Neutrophil Peptide (HNP)-1-4 which are highly homologous. The three principal human defensins, HNP 1-3, are unique to neutrophils and account for about 99 percent of the total defensin content of these cells. Measured amount of defensins is 3-5 mg per million human neutrophils. Activation of neutrophils leads to rapid release of defensins. Thus, only one cell type, neutrophils, may be the source of HNP1-3 measured in plasma and other body fluids during infection and inflammation. In normal plasma low levels of HNP are present ranging from undetectable level to 50-100 ng/ml, while in septic conditions the levels of HNP might be elevated to 10 mg/ml and even more. Activation of neutrophils in blood as occurs during clotting, as well as long storage of anticoagulated blood leads to a release of HNP, thus careful plasma sampling is important for possible detection of HNP. Defensins are relatively resistant to proteolysis, low pH and boiling, but have a tendency to bind to a variety of materials, including plastic and proteins. The HNP 1-3 ELISA shows cross reactivity with Rhesus monkey HNP 1-3. |
| Previous Article No | HK317-02, 182-HK317-02 |
| Product Type | Assay & Detection |
| Range | 156-10,000 pg/ml |
| Research area | Immunology, Microbiology |
| Sensitivity | 156 pg/ml |
| Shipping Information | Room Temperature |
| Size | 2 x 96 det. |
| Species Reactivity | human |
| Stability | Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months. |
| Storage | Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least six months. |
| Technical Specifications | The human HNP1-3 (Neutrophil Defensins) ELISA kit is to be used for the in vitro quantitative determination of human HNP1-3 in plasma and cell culture supernatant samples. |
| Product Page Updated | 2024-04-05T09:42:44.568Z |
