Article No
155-36-180
Specifications
| NCBI Number | 12370 |
| Accession Number | 25090576 |
| Application | ELISA, FC, IHC, WB |
| Article No | 155-36-180 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Clone | 1G12 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | Caspase-8 Antibody [1G12] |
| Supplier | ProSci |
| Entrez Gene ID | 12370 |
| Format | Liquid |
| Gene Symbol | Casp8 |
| Immunogen | Recombinant mouse caspase-8 (p20 subunit). |
| Isotype | IgG1 k |
| Notes | Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p20, respectively, which leads to the generation of the active caspase-8 heterotetramer (p20/p10)2. |
| Alias Names | Apoptotic Cysteine Protease; Apoptotic Protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like Protease |
| Previous Article No | 155-36-180-100 |
| Product Type | Antibodies Primary |
| Protocol | ELISA: (recombinant mouse caspase-8) . Flow Cytometry: (overexpressed in cell lines) . Immunocytochemistry: (see literature reference 2) . Western Blot: (excellent) . Optimal conditions must be determined individually for each application. |
| Purification | >95% (SDS-PAGE) |
| Research area | Cell Death |
| Shipping Information | BLUE ICE |
| Size | 100 ug |
| Source / Host | rat |
| Species Reactivity | mouse |
| Storage | -20°C |
| Substrate / Buffer | Liquid. In PBS containing 0.02% sodium azide. |
| UniProt Number | O89110 |
| Product Page Updated | 2024-01-03T13:34:25.701Z |
