Article No
155-33-082
Specifications
| NCBI Number | 916 |
| Application | FC, IP, WB |
| Article No | 155-33-082 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Clone Type | polyclonal |
| Concentration | 0.2 mg/ml |
| Conjugation | Unconjugated |
| Description | CD3e Antibody |
| Supplier | ProSci |
| Entrez Gene ID | 916 |
| Format | Liquid |
| Gene Symbol | CD3E |
| Immunogen | Amino acids ERPPPVPNPDYEP (156-168) were used as the immunogen for this CD3e antibody. |
| Isotype | IgG |
| Notes | This antibody recognizes the epsilon-chain of CD3 (CD3e), which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the Tcell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the Tcell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the Tcell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the Tcell surface. CD3 antigen is a highly specific marker for Tcells, and is present in majority of Tcell neoplasms. |
| Alias Names | T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E |
| Previous Article No | 155-33-082-100 |
| Product Type | Antibodies Primary |
| Protocol | Flow Cytometry: use the CD3e antibody at 0.5-1 ug/million cells WB: 0.5-1.0 ug/ml IP: 1-2ug/500ug protein lysate The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD3e antibody to be titered up or down for optimal performance. |
| Purification | Protein A Purified |
| Research area | Immunology |
| Shipping Information | BLUE ICE |
| Size | 100 ug |
| Source / Host | rabbit |
| Species Reactivity | cat/feline, chicken, cow/bovine, dog/canine, frog, guinea pig, hamster, horse/equine, human, mammals, mouse, pig/porcine, rabbit, rat, rodent, sheep/ovine |
| Storage | 4°C |
| Substrate / Buffer | PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
| UniProt Number | P07766 |
| Product Page Updated | 2024-01-03T13:34:25.701Z |
