Article No
12-1214-20
MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker)
Anti-MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Recombinant Rabbit Monoclonal Antibody (Clone:MUC1/1887R)
Antibodies Primary
Specifications
| Application | IHC, WB |
| Article No | 12-1214-20 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone | MUC1/1887R |
| Clone Type | monoclonal |
| Conjugation | Unconjugated |
| Description | Anti-MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Recombinant Rabbit Monoclonal Antibody (Clone:MUC1/1887R) |
| Supplier | Abeomics |
| Entrez Gene ID | 4582 |
| Gene Symbol | MUC1 |
| Immunogen | Human milk-fat globule membranes (HMFGM) |
| Isotype | IgG |
| Notes | This MAb reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. It is used as tracer agent in CA15.3 assays. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up-regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion. The dominant epitope of this MAb involves both amino acids as well as sµgar moieties. Its epitope is destroyed by desialylation i.e. treatment with Neuraminidase. |
| Alias Names | Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, Epithelial Membrane Antigen, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface associated, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PE |
| Product Type | Antibodies Primary |
| Protocol | Western Blot (1-2µg/ml); ,Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes) |
| Purification | Protein A/G Purified |
| Shipping Information | Blue Ice |
| Size | 20 µg |
| Source / Host | rabbit |
| Species Reactivity | human |
| Storage | 4°C, -80°C |
| Substrate / Buffer | 200 µg/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| UniProt Number | P15941 |
| Product Page Updated | 2024-02-06T07:35:53.072Z |
