CRISPR/Cas9 Kits & Antibodies

 

EpiQuik CRISPR/Cas9 Assay ELISA Kit (Colorimetric)

Schematic procedure of the EpiQuik™ CRISPR/Cas9 Assay ELISA Kit (Colorimetric).

Suitable for specifically measuring CRISPR-associated protein 9 (Cas9 and dCas9: S. pyogenes) amounts quantitatively with use of purified Cas9 nuclease and whole cell extracts isolated from tissues and cultured cells of various species. The kit has the following advantages:

  • Quick and efficient procedure, which can be finished within 3 hours.
  • Innovative colorimetric assay in high throughput format to quantitatively measure Cas9 protein amount without the need for electrophoresis, especially suitable for screening Cas9-transfected clones. 
  • High sensitivity and specificity. The detection limit is as low as 0.1 ng/well with dynamic range of 0.5-20 ng/well within the indicated amount range of the whole cell extracts. Only recognizes Cas9 (S. pyogenes). 
  • The control is conveniently included for the quantification of Cas9 amount.
  • Strip microplate format makes the assay flexible: manual or high throughput.
  • Simple, reliable, and consistent assay conditions.

    Cat.#P-4060

 

  

EpiQuik CRISPR/SaCas9 (S. aureus) Assay ELISA Kit (Colorimetric)

Schematic procedure of the EpiQuik™ CRISPR/SaCas9 (S. aureus) Assay ELISA Kit (Colorimetric).

The EpiQuik™ CRISPR/SaCas9 (S. aureus) Assay ELISA Kit (Colorimetric) is suitable for specifically measuring CRISPR-associated protein 9 (Cas9 and dCas9: S. aureus) amounts quantitatively with use of purified SaCas9 nuclease and whole cell extracts isolated from tissues and cultured cells of various species. The kit has the following advantages:

  • Quick and efficient procedure, which can be finished within 3 hours.
  • Innovative colorimetric assay in high throughput format to quantitatively measure Cas9 protein amount without the need for electrophoresis, especially suitable for screening Cas9-transfected clones. 
  • High sensitivity and specificity. The detection limit is as low as 0.1 ng/well with dynamic range of 0.5-20 ng/well within the indicated amount range of the whole cell extracts. Only recognizes SaCas9 (S. aureus). 
  • The control is conveniently included for the quantification of SaCas9 amount.
  • Strip-well microplate format makes the assay flexible for manual or high throughput analysis.
  • Simple, reliable, and consistent assay conditions.

Cat. #P-4062

 

 


 

 

CRISPR/Cas9 Monoclonal Antibody [7A9]

Mouse monoclonal antibody raised against CRISPR/Cas9, clone 7A9, generated with synthesized peptide corresponding to sequence of Streptococcus pyogene (S. pyogenes) CRISPR-associated endonuclease Cas9/Csn1. This Anti-Cas9 mAb can detect CRISPR/Cas9 expression in target cells by WB, IF, IP, or ELISA to confirm and verify whether gRNA and Cas9 vectors are successfully transfected.

Cat. #A-9000

 

 


CRISPR/Cas9 (SaCas9) Monoclonal Antibody [6H4]

 

Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde and permeabilized with 0.5% Triton-X at room temperature. After blocking in 2% BSA in PBS, cells were stained with purified CRISPR/Cas9 (SaCas9) Monoclonal Antibody [6H4] (1:1000 diluted) in 1% BSA/PBS at 4°C o/n. After washing, cells were incubated with anti-mouse DyLight594 secondary antibody for 1 hour at room temperature, nuclei were counterstained with Hoechst 33342 and slides were mounted with Vectashield.
Transiently transfected U2OS cells expressing SaCas9-T2A-GFP were fixed with 3.7% formaldehyde and permeabilized with 0.5% Triton-X at room temperature. After blocking in 2% BSA in PBS, cells were stained with purified CRISPR/Cas9 (SaCas9) Monoclonal Antibody [6H4] (1:1000 diluted) in 1% BSA/PBS at 4°C o/n. After washing, cells were incubated with anti-mouse DyLight594 secondary antibody for 1 hour at room temperature, nuclei were counterstained with Hoechst 33342 and slides were mounted with Vectashield. CRISPR/Cas9 (SaCas9) Monoclonal Antibody, Clone 6H4. N-terminal Antibody. Unconjugated. Raised in: Mouse.

Cat. #A-9001