Nordic BioSite is a proud distributor of the whole range of Genetex product range. Genetex has been in the business for 20 years, starting off in the fields of breast cancer biology and prognostic marker research. GeneTex’s product portfolio now cover research areas including cancer, cell biology, epigenetics, immunology, infectious disease, metabolism, neuroscience, signal transduction, stem cell development and zebrafish biology.
GeneTex is committed to the highest standards of product performance through their stringent quality control process.
To meet this mission, they resolve to abide by the validation guidelines proposed by the International Working Group on Antibody Validation (IWGAV) 1 including genetic strategies (see their knockout/knockdown program) and independent antibody strategies (see their side-by-side program).
GeneTex has been conducting side-by-side western blot (WB) and immunohistochemistry (IHC) studies comparing GeneTex reagents to their competitors targeting the same proteins. There goal is to provide highly validated and high-quality GeneTex antibodies to assist you to attain the best possible results with their products.
Epithelial-Mesenchymal Transition (EMT)
Epithelial-mesenchymal transition (EMT) occurs when largely adherent and polarized epithelial cells acquire the phenotype of more mobile mesenchymal cells. EMT not only facilitates morphogenesis during embryonic development but also promotes invasion and metastasis in tumors. Pathological EMT is associated with E-cadherin repression, which contributes to tumor progression. Various oncogenic pathways (involving, for example, TGF-beta, Wnt/beta-catenin, integrins, and Notch) induce EMT via cytoskeletal rearrangement and activation of E-cadherin repressors, including SNAI1, SNAI2, SIP1, ZEB1, and TCF3.
E-cadherin, a calcium-dependent cell-cell adhesion molecule, is a key component of the adherens junctions that are integral in cell adhesion and in maintaining the epithelial cell phenotype. Tumor progression is often associated with the loss of E-cadherin function and the transition to a more motile and invasive phenotype.
Cells that undergo EMT commonly switch from expressing E-cadherin to N-cadherin. The upregulation of N-cadherin expression promotes cell motility and invasion, which is linked to tumor metastasis.
Alpha-smooth muscle actin (alpha-SMA) is the actin isoform that is predominately expressed within vascular smooth muscle cells and plays an important role in fibrogenesis. Alpha-SMA is also considered to be a mesenchymal marker during EMT.
Vimentin is an intermediate filament protein that is characteristically upregulated in cells undergoing EMT. During EMT, cytoskeletal intermediate filaments undergo a significant compositional change from keratin (epithelial cells) to vimentin (mesenchymal cells).
ZEB1 is a zinc finger- and homeodomain-containing transcription factor that binds to the E-box of target promoters. Its binding to the CDH1 promoter leads to the gene’s repression and promotion of tumor invasion and metastasis by inducing EMT.
SNAI1 is a zinc finger transcriptional repressor controlling EMT during embryogenesis and tumor progression. It interacts with several corepressors to repress specific target genes, such as the CDH1 (E-cadherin) gene.
Full product list
|Cat. No.||Product Name||Clonality||Species Reactivity||Application|
|GTX629691||E-Cadherin antibody||Ms mAb||Hu,Ms,Rat||WB, ICC/IF, IHC-P, FACS, IP|
|GTX112975||Cytokeratin 8 antibody||Rb pAb||Hu,Ms||WB, ICC/IF, IHC-P, IP|
|GTX105624||Cytokeratin 18 antibody||Rb pAb||Hu||WB, ICC/IF, IHC-P|
|GTX112666||Cytokeratin 19 antibody||Rb pAb||Hu,Ms,Rat||WB, ICC/IF, IHC-P|
|GTX129931||Desmoglein 3 antibody||Rb pAb||Hu||WB, IP|
|GTX100459||MUC1 antibody||Rb pAb||Hu||WB, ICC/IF, IHC-P, IP|
|GTX108592||ZO-1 antibody||Rb pAb||Hu,Ms||WB, IHC-P|
|GTX100034||alpha Smooth Muscle Actin antibody||Rb pAb||Hu,Ms,Rat,Zfsh||WB, ICC/IF, IHC-P, IHC-Fr|
|GTX112794||Fibronectin antibody||Rb pAb||Hu,Ms||WB, ICC/IF, IHC-P, IP, ELISA|
|GTX130705||Integrin alpha 5 antibody||Rb pAb||Hu,Ms,Rat||WB|
|GTX128839||Integrin beta 1 / CD29 antibody||Rb pAb||Hu,Ms,Rat,Dog||WB, ICC/IF, IHC-P, IP|
|GTX100458||MMP9 antibody||Rb pAb||Hu,Ms,Bov||WB, ICC/IF, IHC-P|
|GTX127345||N-Cadherin antibody||Rb pAb||Hu,Ms,Rat||WB, ICC/IF, IHC-P, IHC-Fr|
|GTX130023||TGF beta 1 antibody||Rb pAb||Hu,Ms||WB|
|GTX100619||Vimentin antibody||Rb pAb||Hu,Ms,Rat||WB, ICC/IF, IHC-P, IHC-Fr, IP|
|GTX633010||beta Catenin antibody||Ms mAb||Hu,Ms||WB, ICC/IF, IHC-P|
|GTX125918||SNAI1 antibody||Rb pAb||Hu,Ms,Rat||WB, ICC/IF, IHC-P, IHC|
|GTX129237||TCF3 antibody||Rb pAb||Hu||WB, ICC/IF, IP|
|GTX127310||Twist1/2 antibody||Rb pAb||Hu,Ms,Rat||WB, IHC-P|
|GTX105278||ZEB1 antibody||Rb pAb||Hu,Ms||WB, ICC/IF, IP, ChIP assay|
|GTX631940||ARID5A antibody||Ms mAb||Hu,Ms||WB, IHC-Fr|
|GTX134542||CD11b antibody||Rb pAb||Ms||WB|
|GTX134588||CDC42 antibody||Rb pAb||Hu,Ms,Rat||WB|
|GTX633917||Dicer antibody||Ms mAb||Hu||WB, ICC/IF|
|GTX132495||GEF-H1 antibody||Rb pAb||Dm||WB|
|GTX134513||GLB1 antibody||Rb pAb||Ms,Rat||WB|
|GTX133372||GSK3 beta antibody||Rb pAb||Hu||WB|
|GTX634834||IKK beta antibody||Ms mAb||Hu||WB|
|GTX133781||IL17A antibody||Rb pAb||Hu,Ms,Rat||WB, IHC-P|
|GTX130278||KDM5A / Jarid1A / RBBP2 antibody||Rb pAb||Hu||WB|
|GTX134234||MEK1 antibody||Rb pAb||Hu,Rat||WB|
|GTX634832||MMP2 antibody||Ms mAb||Hu||WB|
|GTX134086||NMDAR2A antibody||Rb pAb||Ms||WB|
|GTX133368||pan Synuclein antibody||Rb pAb||Ms,Rat||WB, ICC/IF, IHC-P, IHC-Fr|
|GTX634811||Park7 / DJ-1 antibody||Ms mAb||Hu||WB|
|GTX134478||PER2 antibody||Rb pAb||Hu,Ms||WB|
|GTX129275||PERK antibody||Rb pAb||Hu||WB|
|GTX134256||PKC eta antibody||Rb pAb||Ms||WB|
|GTX634796||PKM antibody||Ms mAb||Hu,Ms,Rat||WB|
|GTX634162||PYK2 antibody||Ms mAb||Hu||WB|
|GTX633922||QKI antibody||Ms mAb||Hu,Ms,Rat||WB, IHC-P|
|GTX134122||SLIT1 antibody||Rb pAb||Ms||WB|
|GTX133724||SMARCA2 antibody||Rb pAb||Hu,Ms||WB, ICC/IF, IHC-P|
|GTX633972||Synaptophysin antibody||Ms mAb||Ms,Rat||WB, ICC/IF, IHC-P|
|GTX634809||Tau antibody||Ms mAb||Ms,Rat||WB, ICC/IF|
|GTX134087||TMEM119 antibody||Rb pAb||Ms||IHC-P, IHC-Fr|
|GTX131510||TMEM49 antibody||Rb pAb||Hu||WB|
|GTX134541||Tropomyosin 4 antibody||Rb pAb||Hu||WB|
|GTX134445||TRP2 antibody||Rb pAb||Hu||WB|
|GTX129468||ZIC1 antibody||Rb pAb||Hu||WB|
Join in the '10 ul sample program', where you can get 10 µl for 10 EUR or 100 SEK/DKK/NOK respectively until April 30th 2019!
Use promo code: GTX20 to claim this offer.
As there are many researchers who are influenced by the discontinued supply of polyclonal antibodies, GeneTex and Nordic BioSite is happy to assist researchers to find suitable replacements of high quality and continue the research.
Please note that terms and conditions apply!
Please also note that the offer is only valid for up to max 3 items per customer each month, and those customers providing feedback within one month's time will get a 10% discount code on the same item purchased (valid for 6 months). Use promo code: GTX20 to claim this offer.